IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.
Human/Mouse/Rat IMPA1/IMP1 Antibody
R&D Systems | Catalog # MAB98901
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 984603
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Product Specifications
Immunogen
Synthetic peptide containing human IMPA/IMPA1 Peptide
Accession # P29218
Accession # P29218
Specificity
Detects human IMPA/IMPA1 Peptide in direct ELISAs. Detects human, mouse, and rat IMPA/IMPA1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat IMPA1/IMP1 Antibody
Detection of Human. Mouse, and Rat IMPA1/IMP1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB98901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IMPA1/IMP1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Applications for Human/Mouse/Rat IMPA1/IMP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IMPA1/IMP1
Long Name
Inositol Monophosphatase 1
Alternate Names
IMP, IMP 1, IMP1, IMPA, IMPase 1, MRT59, Myo-Inositol Monophosphatase 1
Gene Symbol
IMPA1
UniProt
Additional IMPA1/IMP1 Products
Product Documents for Human/Mouse/Rat IMPA1/IMP1 Antibody
Product Specific Notices for Human/Mouse/Rat IMPA1/IMP1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars