The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Hamster
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 228611
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Product Specifications
Immunogen
E. coli-derived recombinant human JNK1 isoform 2
Met1-Gln384
Accession # NP_002741
Met1-Gln384
Accession # NP_002741
Specificity
Detects human, mouse, and rat JNK1, expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. Does not detect recombinant JNK2 or JNK3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for JNK1 Antibody (228611)
Detection of human JNK1 by Western Blot.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human, Mouse, and Rat JNK1 by Western Blot.
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK1 were detected at approximately 54 kDa and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for JNK1 Antibody (228611)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line
Sample: CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line
Reviewed Applications
Read 2 reviews rated 5 using MAB1776 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: JNK1
Long Name
C-Jun N-terminal Kinase 1
Alternate Names
MAPK8, PRKM8, SAPK1
Gene Symbol
MAPK8
UniProt
Additional JNK1 Products
Product Documents for JNK1 Antibody (228611)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for JNK1 Antibody (228611)
For research use only
Citations for JNK1 Antibody (228611)
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Customer Reviews for JNK1 Antibody (228611) (2)
5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: whole eye lysate and RetinaSpecies: Human, Rat and MouseVerified Customer | Posted 12/29/2016Human (1), rat (2), wild-type mouse(3), and JNK2 ko mouse (4) retina tissue blots probed for JNK1 (upper panel). Actin reprobing (lower panel) shows sample load.JNK1 antibody was used overnight at 1:2500, followed by peroxidase conjugated 2nd antibody and chemiluminescence detection
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Application: Western BlotSample Tested: Human cancer cell whole cell lysateSpecies: HumanVerified Customer | Posted 10/04/2015JNK1 expression in MDA-MB-231 in response to carboplatin treatment.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
TGF-beta Signaling Pathways
Toll-Like Receptor Signaling Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
