Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Hamster

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 228611
View all JNK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human JNK1 isoform 2
Met1-Gln384
Accession # NP_002741

Specificity

Detects human, mouse, and rat JNK1, expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. Does not detect recombinant JNK2 or JNK3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat JNK1 Antibody

Detection of human JNK1 antibody by Western Blot.

Detection of human JNK1 by Western Blot.

Western blot shows recombinant human JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human, Mouse, and Rat JNK1 antibody by Western Blot.

Detection of Human, Mouse, and Rat JNK1 by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK1 were detected at approximately 54 kDa and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human/Mouse/Rat JNK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB1776 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JNK1

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Entrez Gene IDs

5599 (Human); 26420 (Mouse); 116554 (Rat)

Gene Symbol

MAPK8

UniProt

NP_002741

Additional JNK1 Products

Product Documents for Human/Mouse/Rat JNK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat JNK1 Antibody

For research use only

Citations for Human/Mouse/Rat JNK1 Antibody

Customer Reviews for Human/Mouse/Rat JNK1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

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  • Human/Mouse/Rat JNK1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: whole eye lysate and Retina
    Species: Human, Rat and Mouse
    Verified Customer | Posted 12/29/2016
    Human (1), rat (2), wild-type mouse(3), and JNK2 ko mouse (4) retina tissue blots probed for JNK1 (upper panel). Actin reprobing (lower panel) shows sample load.
    JNK1 antibody was used overnight at 1:2500, followed by peroxidase conjugated 2nd antibody and chemiluminescence detection
    Human/Mouse/Rat JNK1 Antibody MAB1776
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 10/04/2015
    JNK1 expression in MDA-MB-231 in response to carboplatin treatment.
    Human/Mouse/Rat JNK1 Antibody MAB1776

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