Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO by Western Blot.
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Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human Kynurenine 3-Monooxygenase/KMO in direct ELISAs. Detects human, mouse, and rat Kynurenine 3-Monooxygenase/KMO in Western blot.
Recombinant Monoclonal Rabbit IgG Clone # 2493A
Protein A or G purified from cell culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynurenine 3-Monooxygenase/KMO
Accession # O15229
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO by Western Blot. Western blot shows lysates of mouse liver tissue, rat spleen tissue, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kynurenine 3-Monooxygenase/KMO at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Kynurenine 3-Monooxygenase/KMO antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Kynurenine 3-Monooxygenase/KMO in Human Placenta. Kynurenine 3-Monooxygenase/KMO was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynurenine 3-Monooxygenase/KMO

Kynurenine 3-Monooxygenase (KMO), also known as Kynurenine 3-Hydroxylase, is a part of the kynurenine pathway of tryptophan degradation (1). KMO catalyzes the NADPH- and flavin adenine dinucleotide (FAD)-dependent 3-hydroxylation of kynurenine to 3-hydroxykynurenine (3-HK). 3-HK is neurotoxic via the generation of hydrogen peroxide (2) and through the excitotoxic effects of its downstream metabolite quinolinic acid (3). The levels of 3-HK and quinolinic acid are increased in the brain with Alzheimer's disease and Huntington's disease (1). Inhibition of KMO was shown to reverse cognitive and motor deficits in mouse models of those diseases via an increase in neuroprotective kynurenic acid (4). KMO is found in the mitochondrial outer membrane of microglial cells in the brain and dendritic cells and macrophages in the periphery (1). 

  1. Schwarcz, R. et al. (2012) Nat. Rev. Neurosci. 13:465.
  2. Okuda, S. et al. (1996) Proc. Natl. Acad. Sci. 93:12553.
  3. Stone, T.W. and M.N. Perkins. (1981) Eur. J. Pharmacol. 72:411.
  4. Zwilling, D. et al. (2011) Cell 145:863.
Entrez Gene IDs
8564 (Human); 98256 (Mouse); 59113 (Rat)
Alternate Names
KMO; kynurenine 3-monooxygenase (kynurenine 3-hydroxylase); Kynurenine 3Monooxygenase; Kynurenine 3-Monooxygenase

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