Human/Mouse/Rat MuRF1/TRIM63 Antibody

Catalog # Availability Size / Price Qty
AF5366
AF5366-SP

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Detection of Human/Mouse/Rat MuRF1 by Western Blot.
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Citations (11)
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Human/Mouse/Rat MuRF1/TRIM63 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat MuRF1/TRIM63 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MuRF1/TRIM63
Met1-Gly325
Accession # Q969Q1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat MuRF1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat MuRF1 by Western Blot. Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41-44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human MuRF1/TRIM63 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human MuRF1/TRIM63 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1/TRIM63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuRF1/TRIM63

TRIM63 (Tripartite motif-containing protein 63; also MURF-1, SMRZ and RING finger protein 28) is a 41 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is a striated muscle protein that is found in both cytoplasm and nucleus. TRIM63 has multiple finctions, among which are the inhibition of PKC epsilon -mediated cardiomyocyte hypertrophy and the maintenance of skeletal muscle M-line integrity. Human TRIM63 is 353 amino acids (aa) in length. It contains one RING finger domain (aa 23‑82), a B-Box type zinc-finger region (aa 117‑159), a coiled-coil region (aa 207‑269) and a C-terminal COS domain. Isoforms of TRIM63 show one potential alternate start site at Met14, a deletion of aa 105‑132 and a 21 aa substitution for aa 326‑353. Over aa 1‑325, human TRIM63 exhibits 93% aa identity with mouse TRIM63.

Long Name
Tripartite Motif-containing 63
Entrez Gene IDs
84676 (Human); 433766 (Mouse); 140939 (Rat)
Alternate Names
EC 6.3.2.-; IRF; IRFMURF2; Iris RING finger protein; MuRF1; MuRF-1; muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; RING finger protein 28FLJ32380; RNF28; RNF28E3 ubiquitin-protein ligase TRIM63; SMRZ; SMRZMURF-1; Striated muscle RING zinc finger protein; TRIM63; tripartite motif containing 63; tripartite motif-containing 63; Tripartite motif-containing protein 63

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Citations for Human/Mouse/Rat MuRF1/TRIM63 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. &beta-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice
    Authors: M Noguchi, T Kitakaze, Y Kobayashi, K Mukai, N Harada, R Yamaji
    Nutrients, 2020;12(8):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Doxorubicin induces cardiomyocyte apoptosis and atrophy through cyclin-dependent kinase 2-mediated activation of forkhead box O1
    Authors: P Xia, J Chen, Y Liu, M Fletcher, BC Jensen, Z Cheng
    J. Biol. Chem., 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. The Paradoxical Effect of PARP Inhibitor BGP-15 on Irinotecan-Induced Cachexia and Skeletal Muscle Dysfunction
    Authors: DG Campelj, CA Timpani, AC Petersen, A Hayes, CA Goodman, E Rybalka
    Cancers, 2020;12(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes
    Authors: H Shimizu, AD Langenbach, J Huang, K Wang, G Otto, R Geisler, Y Wang, JN Chen
    Elife, 2017;6(0):.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
    Authors: L Zhou, Y Huang, H Xie, X Mei
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy
    Authors: Apichart Suksamrarn
    In Vivo, 2016;30(6):869-877.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury.
    Authors: Files D, Xiao K, Zhang T, Liu C, Qian J, Zhao W, Morris P, Delbono O, Feng X
    PLoS ONE, 2014;9(1):e87587.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of beta-hydroxy-beta-methylbutyrate.
    Authors: Wheatley S, El-Kadi S, Suryawan A, Boutry C, Orellana R, Nguyen H, Davis S, Davis T
    Am J Physiol Endocrinol Metab, 2014;306(1):E91-9.
    Species: Porcine
    Sample Types: Protein
    Applications: Western Blot
  9. TRAF6 inhibition rescues dexamethasone-induced muscle atrophy.
    Authors: Sun, Hualin, Gong, Yanpei, Qiu, Jiaying, Chen, Yanfei, Ding, Fei, Zhao, Qing
    Int J Mol Sci, 2014;15(6):11126-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling.
    Authors: Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M
    Am J Physiol Endocrinol Metab, 2014;306(7):E723-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model.
    Authors: Akkad, Hazem, Corpeno, Rebeca, Larsson, Lars
    PLoS ONE, 2014;9(4):e92622.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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