Human/Mouse/Rat MuRF1/TRIM63 Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat MuRF1/TRIM63 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MuRF1/TRIM63
    Met1-Gly325
    Accession # Q969Q1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat MuRF1 by Western Blot. Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41‑44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human MuRF1/TRIM63 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1/TRIM63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MuRF1/TRIM63

TRIM63 (Tripartite motif-containing protein 63; also MURF-1, SMRZ and RING finger protein 28) is a 41 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is a striated muscle protein that is found in both cytoplasm and nucleus. TRIM63 has multiple finctions, among which are the inhibition of PKC epsilon -mediated cardiomyocyte hypertrophy and the maintenance of skeletal muscle M-line integrity. Human TRIM63 is 353 amino acids (aa) in length. It contains one RING finger domain (aa 23‑82), a B-Box type zinc-finger region (aa 117‑159), a coiled-coil region (aa 207‑269) and a C-terminal COS domain. Isoforms of TRIM63 show one potential alternate start site at Met14, a deletion of aa 105‑132 and a 21 aa substitution for aa 326‑353. Over aa 1‑325, human TRIM63 exhibits 93% aa identity with mouse TRIM63.

  • Long Name:
    Tripartite Motif-containing 63
  • Entrez Gene IDs:
    84676 (Human); 433766 (Mouse); 140939 (Rat)
  • Alternate Names:
    EC 6.3.2.-; IRF; IRFMURF2; Iris RING finger protein; MuRF1; MuRF-1; muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; RING finger protein 28FLJ32380; RNF28; RNF28E3 ubiquitin-protein ligase TRIM63; SMRZ; SMRZMURF-1; Striated muscle RING zinc finger protein; TRIM63; tripartite motif containing 63; tripartite motif-containing 63; Tripartite motif-containing protein 63
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes
    Authors: H Shimizu, AD Langenbach, J Huang, K Wang, G Otto, R Geisler, Y Wang, JN Chen
    Elife, 2017;6(0):.
    Species: Zebrafish
    Sample Type: Tissue Homogenates
    Application: WB
  2. The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury.
    Authors: Files D, Xiao K, Zhang T, Liu C, Qian J, Zhao W, Morris P, Delbono O, Feng X
    PLoS ONE, 2014;9(1):e87587.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of beta-hydroxy-beta-methylbutyrate.
    Authors: Wheatley S, El-Kadi S, Suryawan A, Boutry C, Orellana R, Nguyen H, Davis S, Davis T
    Am J Physiol Endocrinol Metab, 2014;306(1):E91-9.
    Species: Porcine
    Sample Type: Protein
    Application: WB
  4. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling.
    Authors: Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M
    Am J Physiol Endocrinol Metab, 2014;306(7):E723-39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. TRAF6 inhibition rescues dexamethasone-induced muscle atrophy.
    Authors: Sun, Hualin, Gong, Yanpei, Qiu, Jiaying, Chen, Yanfei, Ding, Fei, Zhao, Qing
    Int J Mol Sci, 2014;15(6):11126-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model.
    Authors: Akkad, Hazem, Corpeno, Rebeca, Larsson, Lars
    PLoS ONE, 2014;9(4):e92622.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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