Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 683211
View all NEDD4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NEDD4
Asn211-Gly349 (predicted)
Accession # NP_006145

Specificity

Detects human NEDD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) NEDD8 or rhNEDD9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat NEDD4 Antibody

Detection of Human, Mouse, and Rat NEDD4 antibody by Western Blot.

Detection of Human, Mouse, and Rat NEDD4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human NEDD4 Monoclonal Antibody (Catalog # MAB6218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEDD4 at approximately 118 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of NEDD4 by Western Blot

Detection of NEDD4 by Western Blot

UL42 identified as a hub of E3 destruction by a combination of interactome and degradation data.US10 interacts with LRFN3, which is rapidly downregulated from the PM during HCMV infection. (D) Validation of interaction between UL42 and NEDD4 (left panel) and NEDD4L (right panel) by co-IP, conducted as described in Figure 3. HEK293T cells were transiently transfected with the indicated plasmids, one expressing the C-terminally V5-tagged viral protein and the other expressing C-terminally HA-tagged NEDD4 or NEDD4L. These proteins were detected with anti-V5 and anti-HA. This figure is representative of n = 2 experiments (NEDD4); n = 1 experiment (NEDD4L). Expected sizes: NEDD4: 104–149 kDa; NEDD4L: 96–111 kDa; UL42: 14 kDa; UL34: 45 kDa; CANX: 72 kDa. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31873071), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NEDD4 by Western Blot

Detection of NEDD4 by Western Blot

UL42 identified as a hub of E3 destruction by a combination of interactome and degradation data.US10 interacts with LRFN3, which is rapidly downregulated from the PM during HCMV infection. (E) UL42 was sufficient to degrade NEDD4. HFFF-TERTs expressing UL42 or controls were lysed and immunoblotted as indicated. Anti-NEDD4 was used to detect endogenous NEDD4. This figure is representative of n = 1 experiment. Expected sizes: NEDD4: 104–149 kDa; UL42: 14 kDa; UL34: 45 kDa; CANX: 72 kDa. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31873071), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat NEDD4 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD4

NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1; also RPF1) is a 115-120 kDa member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900 amino acids (aa) in length. It contains one PKC region (aa 20-124), four WW domains (aa 196-505) and one E3 HECT domain (aa 543-897). Caspase cleavage after Asp197 generates a 95 kDa C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516 aa substitution for aa 1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa 170-185. Over aa 211-349, human NEDD4-1 shares 83% aa identity to mouse NEDD4-1 and less than 25% aa identity with human NEDD4-2.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Entrez Gene IDs

4734 (Human); 17999 (Mouse); 25489 (Rat)

Gene Symbol

NEDD4

UniProt

NP_006145

Additional NEDD4 Products

Product Documents for Human/Mouse/Rat NEDD4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat NEDD4 Antibody

For research use only

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

Citations for Human/Mouse/Rat NEDD4 Antibody

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