NEDD8 Antibody (584606)

R&D Systems | Catalog # MAB4936

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 584606
View all NEDD8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NEDD8
Leu2-Gly77
Accession # Q15843

Specificity

Detects human, mouse, and rat NEDD8 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for NEDD8 Antibody (584606)

Detection of Human, Mouse, and Rat NEDD8 antibody by Western Blot.

Detection of Human, Mouse, and Rat NEDD8 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB4936) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEDD8 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for NEDD8 Antibody (584606)

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD8

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6‑8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81 amino acids (aa) in length and contains one ubiquitin-like domain (aa 1‑65), and a 4‑5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Entrez Gene IDs

4738 (Human); 18002 (Mouse); 25490 (Rat)

Gene Symbol

NEDD8

UniProt

Q15843 (Human)

Additional NEDD8 Products

Product Documents for NEDD8 Antibody (584606)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for NEDD8 Antibody (584606)

For research use only

Citations for NEDD8 Antibody (584606)

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