Human/Mouse/Rat NEDD8 Antibody Summary
Accession # Q15843
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat NEDD8 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat NEDD8 Monoclonal Antibody (Catalog # MAB4936) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEDD8 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6‑8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81 amino acids (aa) in length and contains one ubiquitin-like domain (aa 1‑65), and a 4‑5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.
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