Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Peroxiredoxin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 1
Met1-Lys199
Accession # Q06830

Specificity

Detects human, mouse and rat Peroxiredoxin 1 in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Peroxiredoxin 3 or 4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 1 Antibody

Detection of Human/Mouse/Rat Peroxiredoxin 1 antibody by Western Blot.

Detection of Human/Mouse/Rat Peroxiredoxin 1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Detection of Human Peroxiredoxin 1 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (PC‑3) (NBP2-49856), and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Western Blot Shows Human Peroxiredoxin 1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Human Peroxiredoxin 1 antibody by Simple WesternTM.

Detection of Human Peroxiredoxin 1 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 1 at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3488) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Peroxiredoxin 1 Antibody

Application
Recommended Usage

Knockout Validated

Peroxiredoxin 1 is specificlly detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. 

Simple Western

2-10 µg/mL
Sample: Exosome Standard (HT-29) (Catalog # NBP3-11685), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line

Western Blot

0.2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxiredoxin 1

Human Peroxiredoxin 1 (Prx-1 or PRDX1; also Thioredoxin Peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length, and has two catalytic cysteines, one at Cys52, and a second at Cys173. Prx-1 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms. One shows a premature truncation after aa 171, while the second shows a deletion of aa 21 - 121. Human Prx-1 shows 96% and 98% amino acid identity to mouse and rat Prx-1, respectively.

Alternate Names

NKEF-A, PRDX1, PRX1, TDPX2

Entrez Gene IDs

5052 (Human); 18477 (Mouse); 117254 (Rat)

Gene Symbol

PRDX1

UniProt

Q06830

Additional Peroxiredoxin 1 Products

Product Documents for Human/Mouse/Rat Peroxiredoxin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 1 Antibody

For research use only

Citations for Human/Mouse/Rat Peroxiredoxin 1 Antibody

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