Human/Mouse/Rat Peroxiredoxin 2 Antibody Summary
Accession # P32119
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Peroxiredoxin 2 Monoclonal Antibody (Catalog # MAB3489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 2
Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.
Citation for Human/Mouse/Rat Peroxiredoxin 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress.
Authors: Van Brussel, Ilse, Schrijvers, Dorien M, Martinet, Wim, Pintelon, Isabel, Deschacht, Maartje, Schnorbusch, Kathy, Maes, Louis, Bosmans, Johan M, Vrints, Christia, Adriaensen, Dirk, Cos, Paul, Bult, Hidde
PLoS ONE, 2012;7(8):e43357.
Sample Types: Cell Lysates
Applications: Western Blot
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