Peroxiredoxin 2 Antibody (477719)

R&D Systems | Catalog # MAB3489

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 477719
View all Peroxiredoxin 2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119

Specificity

Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is observed. In direct ELISAs, approximately 15%-25% cross-reactivity with rhPeroxiredoxin 1 and 4 is observed, and less than 5% cross-reactivity with rhPeroxiredoxin 3, 5, or 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Peroxiredoxin 2 Antibody (477719)

Detection of Human/Mouse/Rat Peroxiredoxin 2 antibody by Western Blot.

Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Peroxiredoxin 2 Monoclonal Antibody (Catalog # MAB3489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Peroxiredoxin 2 Antibody (477719)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB3489 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxiredoxin 2

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Alternate Names

NKEF-B, PRDX2, PRX2, TDPX1, TSA

Entrez Gene IDs

7001 (Human); 21672 (Mouse); 29338 (Rat)

Gene Symbol

PRDX2

UniProt

P32119

Additional Peroxiredoxin 2 Products

Product Documents for Peroxiredoxin 2 Antibody (477719)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Peroxiredoxin 2 Antibody (477719)

For research use only

Related Research Areas

Cancer Biomarkers
Oxidative Stress

Citations for Peroxiredoxin 2 Antibody (477719)

Customer Reviews for Peroxiredoxin 2 Antibody (477719) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat Peroxiredoxin 2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Peroxiredoxin 2 Antibody (477719) MAB3489
  • Human/Mouse/Rat Peroxiredoxin 2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018
  • Human/Mouse/Rat Peroxiredoxin 2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

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