Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat PLC-gamma 1 when phosphorylated at Y783.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1016D
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human/mouse/rat PLC-gamma 1 Y783 site.
    Accession # P19174
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    3-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Phospho-PLC‑ gamma 1 (Y783) in A431 Human Cell Line. PLC‑ gamma 1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF
(Catalog # 236-EG) using Rabbit Anti-Human Phospho-PLC‑ gamma 1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 1
Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones, and phosphorylation at Tyr783 is a widely used indicator of activation. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.
  • Long Name:
    Phospholipase C gamma 1
  • Entrez Gene IDs:
    5335 (Human); 18803 (Mouse); 25738 (Rat)
  • Alternate Names:
    EC 3.1.4.11; gamma 1 (formerly subtype 148); monophosphatidylinositol phosphodiesterase; NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; PLC148; PLC-148; PLCG1; PLCgamma 1; PLC-gamma 1; PLC-gamma-1; PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; PPLCA; triphosphoinositide phosphodiesterase
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