Human/Mouse/Rat Phospho-PLC-gamma 1 (Y783) Antibody

Phospho-PLC‑ gamma 1 (Y783) in A431 Human Cell Line. PLC- gamma1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse PLC-gamma 1 by Western Blot Cathelicidin-dependent signaling in platelets. a–i LL-37 induced signaling in isolated human platelets. a–c Flow cytometry analysis of platelet P-selectin surface expression in the presence of a the calcium chelator BAPTA (n = 5) or a phospholipase C inhibitor (U-73122, n = 6), b pertussis or cholera toxin to inhibit G-protein signaling (n = 4), or c inhibitors of tyrosine kinases Src-family kinases (Dasatinib, n = 7) and Syk (R406, n = 5). d Representative western blots of phosphorylated Src-family kinase and phosphorylated Syk upon incubation of platelets with LL-37. Collagen was used as positive control for tyrosine kinase phosphorylation, beta -actin served as loading control. Images are representative of three independent blots. e–i Flow cytometry analysis of LL-37 platelet P-selectin surface expression in the presence of e STAT3 small molecule inhibitor (Stattic, n = 6), f GPVI antibody (HGP5C4, n = 9), g GPIIb/IIIa antibody (Abciximab or Tirofiban, n = 4), h formyl-peptid-receptor (FPR1 or FPR2) antibody, and i inhibitors against the purinergic P2X7-receptor (Boc-MLF 10, WRW4 or A438079, n = 4). j–l CRAMP-induced signaling in isolated mouse platelets. j P-selectin surface expression in the presence of a GPVI depleting antibody (JAQ1, n = 6). k P-selectin surface expression and l phospholipase C phosphorylation in platelet lysates from platelet-specific Syk-deficient mice and respective littermates after stimulation with CRAMP (flow cytometry: n = 10 Syk−/− and n = 5 Syk+/+ animals, western blot analysis n = 5 each). Graphs show mean and SEM. P-values were determined by unpaired (a, f, j–l) or paired (c, e, h) t-test Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29670076), licensed under a CC-BY license. Not internally tested by R&D Systems.