PP2C epsilon/PPM1L Antibody (700612)

R&D Systems | Catalog # MAB6914

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 700612
View all PP2C epsilon/PPM1L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2C epsilon /PPPM1L
Asp43-Arg178
Accession # Q5SGD2

Specificity

Detects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PP2C epsilon/PPM1L Antibody (700612)

Detection of Human, Mouse, and Rat PP2C e/PPM1L antibody by Western Blot.

Detection of Human, Mouse, and Rat PP2C epsilon /PPM1L by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C e/PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PP2C epsilon/PPM1L Antibody (700612)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line, Nb2‑11 rat lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C epsilon/PPM1L

PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon.

Long Name

Protein Phosphatase 2C alpha

Alternate Names

PP2CE, PPM1L

Entrez Gene IDs

151742 (Human); 242083 (Mouse); 310506 (Rat)

Gene Symbol

PPM1L

UniProt

Q5SGD2

Additional PP2C epsilon/PPM1L Products

Product Documents for PP2C epsilon/PPM1L Antibody (700612)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PP2C epsilon/PPM1L Antibody (700612)

For research use only

Citations for PP2C epsilon/PPM1L Antibody (700612)

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway
MAPK Signaling: Oxidative Stress Pathway Thumbnail