Human/Mouse/Rat PP2C epsilon /PPM1L Antibody Summary
Accession # Q5SGD2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat PP2C epsilon /PPM1L by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C e/PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C epsilon/PPM1L
PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon.
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