PTP-MEG2 Antibody (291835)

Detection of Human/Mouse/Rat PTP-MEG2 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, TS1 mouse helper T cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PTP-MEG2 Monoclonal Antibody (Catalog # MAB2668) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTP-MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PTP-MEG2 by Western Blot

MEG2 is downregulated in human gastric cancer and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells. a and b Western blot analysis of MEG2 protein in 20 paired human gastric cancer specimens (GC) and normal adjacent gastric tissue (GN) samples. a representative image; b quantitative analysis. c Western blot analysis of MEG2 protein in 5 human GC cell lines and 1 human gastric epithelial cell line. d Western blot analysis of MEG2 protein in MGC803 cells transfected with MEG2 siRNA, control siRNA, MEG2 plasmid and control plasmid. e Cell proliferation assays were performed after the transfection of MGC803 cells with MEG2 siRNA and control siRNA in equal doses. f Cell proliferation assays were performed after the transfection with MEG2 plasmid and control plasmid in equal doses. g and h Transwell migration assays were performed after the transfection with MEG2 siRNA, control siRNA, MEG2 plasmid and control plasmid. g representative images; h quantitative analysis. * P < 0.05; ** P < 0.01; *** P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28747184), licensed under a CC-BY license. Not internally tested by R&D Systems.