Human/Mouse/Rat PTP-MEG2 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat PTP-MEG2.
  • Source
    Monoclonal Mouse IgG2B Clone # 291835
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PTP-MEG2
    Glu2-Gln593
    Accession # P43378
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed human granulocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat PTP-MEG2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, TS1 mouse helper T cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PTP-MEG2 Monoclonal Antibody (Catalog # MAB2668) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTP-MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP-MEG2

Megakaryocyte protein tyrosine phosphatase 2 (PTP-MEG2), also known as PTPN9, contains an N-terminal domain homologous to the yeast retinaldehyde binding domain Sec14p and a C-terminal phosphatase domain. The Sec14p domain targets PTP-MEG2 to the membrane of secretory vesicles, where it promotes vesicle fusion. Elevated levels of membrane-bound PTP-MEG2 activity in erythroid progenitor cells are observed in polycythemia vera and may affect responsiveness to colony stimulating factors.

  • Long Name:
    Megakaryocyte Protein Tyrosine Phosphatase 2
  • Entrez Gene IDs:
    5780 (Human); 56294 (Mouse); 266611 (Rat)
  • Alternate Names:
    EC 3.1.3.48; MEG2; protein tyrosine phosphatase, non-receptor type 9; Protein-tyrosine phosphatase MEG2; PTPase MEG2; PTPase-MEG2; PTPMEG2; PTP-MEG2; PTPN9; tyrosine-protein phosphatase non-receptor type 9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. MEG2 is regulated by miR-181a-5p and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells
    Authors: Z Liu, F Sun, Y Hong, Y Liu, M Fen, K Yin, X Ge, F Wang, X Chen, W Guan
    Mol. Cancer, 2017;16(1):133.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. miR-96 promotes cell proliferation, migration and invasion by targeting PTPN9 in breast cancer
    Sci Rep, 2016;6(0):37421.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells.
    Authors: Huang X, Gschweng E, Van Handel B
    Blood, 2011;117(7):2157-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis.
    Authors: Hao Q, Wang L, Zhao ZJ, Tang H
    J. Biol. Chem., 2009;284(2):799-806.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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