Human/Mouse/Rat Rab11A Antibody

Catalog # Availability Size / Price Qty
MAB8978
MAB8978-SP
Detection  of Human, Mouse, and Rat Rab11A by Western Blot.
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Human/Mouse/Rat Rab11A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Rab-11A in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 918009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Rab-11A
Met1-Glu162
Accession # P62491
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human, Mouse, and Rat Rab11A by Western Blot. View Larger

Detection of Human, Mouse, and Rat Rab11A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rab11A Monoclonal Antibody
(Catalog # MAB8978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rab11A

Ras-related protein Rab11A is a 25 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab family members cycle between an inactive GDP-bound form and an active GTP-bound form that recruits to membranes various downstream effectors directly responsible for vesicle formation, migration and fusion.  Rab11A is ubiquitously expressed, with Northern blot analysis detecting highest levels in heart, and lowest levels in liver.  Human Rab11A is 216 amino acids (aa) in length, with residues 214-216 removed in its mature form.  Over aa 1-162, human Rab11A shares 100% identity with mouse and rat Rab11A.

Long Name
RAs Genes from Brain Protein 11A
Entrez Gene IDs
8766 (Human); 53869 (Mouse); 81830 (Rat)
Alternate Names
MGC1490; RAB 11A, member oncogene family; RAB11; Rab-11; Rab11A; RAB11A, member RAS oncogene family; YL8; YL8ras-related protein Rab-11A

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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