Rab11A Antibody (918009)

R&D Systems | Catalog # MAB8978

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 918009
View all Rab11A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rab-11A
Met1-Glu162
Accession # P62491

Specificity

Detects human Rab-11A in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rab11A Antibody (918009)

Detection of Human, Mouse, and Rat Rab11A antibody by Western Blot.

Detection of Human, Mouse, and Rat Rab11A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rab11A Monoclonal Antibody (Catalog # MAB8978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rab11A Antibody (918009)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB8978 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rab11A

Ras-related protein Rab11A is a 25 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab family members cycle between an inactive GDP-bound form and an active GTP-bound form that recruits to membranes various downstream effectors directly responsible for vesicle formation, migration and fusion.  Rab11A is ubiquitously expressed, with Northern blot analysis detecting highest levels in heart, and lowest levels in liver.  Human Rab11A is 216 amino acids (aa) in length, with residues 214-216 removed in its mature form.  Over aa 1-162, human Rab11A shares 100% identity with mouse and rat Rab11A.

Long Name

RAs Genes from Brain Protein 11A

Alternate Names

Rab-11, YL8

Entrez Gene IDs

8766 (Human); 53869 (Mouse); 81830 (Rat)

Gene Symbol

RAB11A

UniProt

P62491

Additional Rab11A Products

Product Documents for Rab11A Antibody (918009)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rab11A Antibody (918009)

For research use only

Related Research Areas

Epithelial Cell Polarity

Citations for Rab11A Antibody (918009)

Customer Reviews for Rab11A Antibody (918009) (2)

3.5 out of 5
2 Customer Ratings
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  • Human/Mouse/Rat Rab11A Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 06/13/2019
    The antibody cannot properly label the typical Rab11 localisation at the Golgi area.
    Rab11A Antibody (918009) MAB8978
  • Human/Mouse/Rat Rab11A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 Cell Lysate
    Species: Human
    Verified Customer | Posted 01/20/2017
    50 ug whole cell lysate of scrambled shRNA (Ctrl) or rab11a shRNA MDA-MB-231 cells. Experimenter technical error on blot.
    a few non-specific bands above 50kDa.
    Rab11A Antibody (918009) MAB8978

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