Detection of Human, Mouse, and Rat Rab11A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rab11A Monoclonal Antibody (Catalog # MAB8978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
protein Rab11A is a 25 kDa member of the Rab family of small GTPases. With more than 40 family members, Rab
proteins are regulators of vesicular transport, and their number reflects the
complexity of vesicle trafficking. Rab
family members cycle between an inactive GDP-bound form and an active GTP-bound
form that recruits to membranes various downstream effectors directly
responsible for vesicle formation, migration and fusion. Rab11A is ubiquitously expressed, with
Northern blot analysis detecting highest levels in heart, and lowest levels in
liver. Human Rab11A is 216 amino acids (aa)
in length, with residues 214-216 removed in its mature form. Over aa 1-162, human Rab11A shares 100%
identity with mouse and rat Rab11A.
RAs Genes from Brain Protein 11A
Entrez Gene IDs:
8766 (Human); 53869 (Mouse); 81830 (Rat)
MGC1490; RAB 11A, member oncogene family; RAB11; Rab-11; RAB11A, member RAS oncogene family; YL8; YL8ras-related protein Rab-11A
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