Human/Mouse/Rat Rap1A/B Antibody Summary
Accession # P62834
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Rap1A/B by Western Blot. Western blot shows recombinant human Rap1A, Rap1B, Rap2A, and Rap2B (1 ng/lane). PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human/Mouse/Rat Rap1A/B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Ras-related proteins Rap1A and Rap1B share 95% identity and together with Rap2A and Rap2B constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Rap proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Rap1 counteracts the mitogenic function of Ras, possibly through competitive interactions with Ras GAPs and Raf, and is implicated in the regulation of integrins.
Citation for Human/Mouse/Rat Rap1A/B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival
Authors: LW Wang, Z Wang, I Ersing, L Nobre, R Guo, S Jiang, S Trudeau, B Zhao, MP Weekes, BE Gewurz
PLoS Pathog., 2019;15(9):e1008030.
Sample Types: Cell Lysates
Applications: Western Blot
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