Human/Mouse/Rat TC-PTP Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat TC‑PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 252294
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TC-PTP
    Pro2-Asn314
    Accession # P17706
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat TC‑PTP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC‑PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC‑PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TC-PTP

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

  • References:
    1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
    2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
    3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
    4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
    5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
    6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
    7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
    8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
    9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
  • Long Name:
    T Cell Protein Tyrosine Phosphatase
  • Entrez Gene IDs:
    5771 (Human); 19255 (Mouse)
  • Alternate Names:
    protein tyrosine phosphatase, non-receptor type 2; PTP2; PTPN2; PTPTPTN2; T-cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TCELLPTP; TCPTP; TC-PTP; TCPTPEC 3.1.3.48; tyrosine-protein phosphatase non-receptor type 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development
    Authors: H Lee, M Kim, M Baek, LD Morales, IS Jang, TJ Slaga, J DiGiovanni, DJ Kim
    Sci Rep, 2017;7(0):45077.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
    Authors: Zhou W, Jeyaraman K, Yusoff P, Shenolikar S
    J Biol Chem, 2013;288(46):33146-55.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation.
    Authors: Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Orntoft TF, Eizirik DL
    J. Biol. Chem., 2011;286(2):929-41.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.
    Authors: Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
    J. Biol. Chem., 2010;285(51):40114-24.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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