Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 252294
View all TC-PTP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TC-PTP
Pro2-Asn314
Accession # P17706

Specificity

Detects human, mouse, and rat TC‑PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat TC‑PTP Antibody

Detection of Human/Mouse/Rat TC‑PTP antibody by Western Blot.

Detection of Human/Mouse/Rat TC‑PTP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC-PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC-PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse TC-PTP/PTPN2 by Western Blot

Detection of Mouse TC-PTP/PTPN2 by Western Blot

Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 (p-STAT3). (A) 3PC keratinocytes were treated with 10, 20, 50 and 100 nM of TPA for 1 h. (B) 3PC keratinocytes were cultured in the presence of 5, 10, 20 and 50 μM Na3VO4 for 6 h. (C) 3PC keratinocyte were cultured with 50 μM of Na3VO4 for 2 h before treatment with 100 nM TPA. Cells were collected 6 h after TPA treatment. (D) 3PC keratinocytes were transfected with siRNA specific for Ptpn2. Cells were collected 1 h after TPA (100 nM) treatment. (E) 3PC keratinocytes were treated with 20, 50 and 100 nM of TPA for 1 h. Cells were collected at the indicated time after TPA treatment. (F–G) Control (scrambled) and TC-PTP-knockdown keratinocytes were treated with TPA (20 nM) for 1 h. Following TPA treatment, cells were collected at the indicated time. (G) *At 3 h of incubation, control and TC-PTP-knockdown keratinocytes were re-treated with TPA for 1 h. Cells were then collected 4 h after second TPA treatment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28322331), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TC-PTP/PTPN2 by Western Blot

Detection of Mouse TC-PTP/PTPN2 by Western Blot

Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 (p-STAT3). (A) 3PC keratinocytes were treated with 10, 20, 50 and 100 nM of TPA for 1 h. (B) 3PC keratinocytes were cultured in the presence of 5, 10, 20 and 50 μM Na3VO4 for 6 h. (C) 3PC keratinocyte were cultured with 50 μM of Na3VO4 for 2 h before treatment with 100 nM TPA. Cells were collected 6 h after TPA treatment. (D) 3PC keratinocytes were transfected with siRNA specific for Ptpn2. Cells were collected 1 h after TPA (100 nM) treatment. (E) 3PC keratinocytes were treated with 20, 50 and 100 nM of TPA for 1 h. Cells were collected at the indicated time after TPA treatment. (F–G) Control (scrambled) and TC-PTP-knockdown keratinocytes were treated with TPA (20 nM) for 1 h. Following TPA treatment, cells were collected at the indicated time. (G) *At 3 h of incubation, control and TC-PTP-knockdown keratinocytes were re-treated with TPA for 1 h. Cells were then collected 4 h after second TPA treatment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28322331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat TC‑PTP Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TC-PTP

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

References

  1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
  2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
  3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
  4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
  5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
  6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
  7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
  8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
  9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.

Long Name

T Cell Protein Tyrosine Phosphatase

Alternate Names

PTP2, PTPN2, TCPTP

Entrez Gene IDs

5771 (Human); 19255 (Mouse)

Gene Symbol

PTPN2

UniProt

P17706

Additional TC-PTP Products

Product Documents for Human/Mouse/Rat TC‑PTP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat TC‑PTP Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human/Mouse/Rat TC‑PTP Antibody

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