Human/Mouse/Rat TC-PTP Antibody

Catalog # Availability Size / Price Qty
MAB1930
MAB1930-SP
Detection of Human/Mouse/Rat TC‑PTP by Western Blot.
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Product Details
Citations (7)
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Human/Mouse/Rat TC-PTP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat TC‑PTP in Western blots. In Western blots, no cross-reactivity with PTP1B is observed.
Source
Monoclonal Mouse IgG2A Clone # 252294
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TC-PTP
Pro2-Asn314
Accession # P17706
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat TC‑PTP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat TC‑PTP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat TC-PTP Monoclonal Antibody (Catalog # MAB1930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and Recombinant Human TC-PTP aa 2-314 (Catalog # 1930-PT) (1 ng/lane) were included. A specific band for TC-PTP was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse TC-PTP/PTPN2 by Western Blot View Larger

Detection of Mouse TC-PTP/PTPN2 by Western Blot Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 (p-STAT3). (A) 3PC keratinocytes were treated with 10, 20, 50 and 100 nM of TPA for 1 h. (B) 3PC keratinocytes were cultured in the presence of 5, 10, 20 and 50 μM Na3VO4 for 6 h. (C) 3PC keratinocyte were cultured with 50 μM of Na3VO4 for 2 h before treatment with 100 nM TPA. Cells were collected 6 h after TPA treatment. (D) 3PC keratinocytes were transfected with siRNA specific for Ptpn2. Cells were collected 1 h after TPA (100 nM) treatment. (E) 3PC keratinocytes were treated with 20, 50 and 100 nM of TPA for 1 h. Cells were collected at the indicated time after TPA treatment. (F–G) Control (scrambled) and TC-PTP-knockdown keratinocytes were treated with TPA (20 nM) for 1 h. Following TPA treatment, cells were collected at the indicated time. (G) *At 3 h of incubation, control and TC-PTP-knockdown keratinocytes were re-treated with TPA for 1 h. Cells were then collected 4 h after second TPA treatment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28322331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse TC-PTP/PTPN2 by Western Blot View Larger

Detection of Mouse TC-PTP/PTPN2 by Western Blot Effects of TC-PTP deficiency on STAT3 dephosphorylation in keratinocytes following TPA treatment.Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for STAT3 and phosphorylated STAT3 (p-STAT3). (A) 3PC keratinocytes were treated with 10, 20, 50 and 100 nM of TPA for 1 h. (B) 3PC keratinocytes were cultured in the presence of 5, 10, 20 and 50 μM Na3VO4 for 6 h. (C) 3PC keratinocyte were cultured with 50 μM of Na3VO4 for 2 h before treatment with 100 nM TPA. Cells were collected 6 h after TPA treatment. (D) 3PC keratinocytes were transfected with siRNA specific for Ptpn2. Cells were collected 1 h after TPA (100 nM) treatment. (E) 3PC keratinocytes were treated with 20, 50 and 100 nM of TPA for 1 h. Cells were collected at the indicated time after TPA treatment. (F–G) Control (scrambled) and TC-PTP-knockdown keratinocytes were treated with TPA (20 nM) for 1 h. Following TPA treatment, cells were collected at the indicated time. (G) *At 3 h of incubation, control and TC-PTP-knockdown keratinocytes were re-treated with TPA for 1 h. Cells were then collected 4 h after second TPA treatment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28322331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TC-PTP

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

References
  1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
  2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
  3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
  4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
  5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
  6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
  7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
  8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
  9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
Long Name
T Cell Protein Tyrosine Phosphatase
Entrez Gene IDs
5771 (Human); 19255 (Mouse)
Alternate Names
protein tyrosine phosphatase, non-receptor type 2; PTP2; PTPN2; PTPTPTN2; T-cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TCELLPTP; TCPTP; TC-PTP; TCPTPEC 3.1.3.48; tyrosine-protein phosphatase non-receptor type 2

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Citations for Human/Mouse/Rat TC-PTP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The EGFR phosphatase RPTP gamma is a redox‐regulated suppressor of promigratory signaling
    Authors: Maitreyi S Joshi, Angel Stanoev, Jan Huebinger, Birga Soetje, Veronika Zorina, Lisaweta Ro beta mannek et al.
    The EMBO Journal
  2. Multiple mechanisms for TRAF3-mediated regulation of the T cell costimulatory receptor GITR
    Authors: H Li, BS Hostager, T Arkee, GA Bishop
    The Journal of Biological Chemistry, 2021-08-18;0(0):101097.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development
    Authors: H Lee, M Kim, M Baek, LD Morales, IS Jang, TJ Slaga, J DiGiovanni, DJ Kim
    Sci Rep, 2017-03-21;7(0):45077.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
    Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A
    J Clin Invest, 2014-07-01;124(8):3295-310.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  5. Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
    Authors: Zhou W, Jeyaraman K, Yusoff P, Shenolikar S
    J Biol Chem, 2013-10-03;288(46):33146-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation.
    Authors: Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Orntoft TF, Eizirik DL
    J. Biol. Chem., 2010-10-27;286(2):929-41.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.
    Authors: Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
    J. Biol. Chem., 2010-10-12;285(51):40114-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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