Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 237020
View all VHR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VHR
Ser2-Pro185
Accession # P51452

Specificity

Detects human, mouse, and rat VHR in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat VHR Antibody

Detection of Human, Mouse, and Rat VHR antibody by Western Blot.

Detection of Human, Mouse, and Rat VHR by Western Blot.

Western blot shows lysates of TE-85 human osteogenic sarcoma cell line, TF-1 human erythroleukemic cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/ Rat VHR Monoclonal Antibody (Catalog # MAB1654) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human VHR (Catalog # 1654-VH) (2 ng) was included. A specific band for VHR was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat VHR Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: TE-85 human osteogenic sarcoma cell line, TF-1 human erythroleukemic cell line, and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VHR

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

References

  1. Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
  2. Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
  3. Alonso, A. et al. (2003) Nat. Immunol. 4:44.
  4. Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
  5. Yuvaniyama, J. et al. (1996) Science 272:1328.
  6. Ueda, K. et al. (2002) FEBS Lett. 525:48.

Long Name

Vaccinia Virus VH1-related Phosphatase

Alternate Names

DUSP3

Entrez Gene IDs

1845 (Human)

Gene Symbol

DUSP3

UniProt

P51452

Additional VHR Products

Product Documents for Human/Mouse/Rat VHR Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat VHR Antibody

For research use only

Related Research Areas

Intracellular Kinases

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