Semaphorin 3E Antibody

R&D Systems | Catalog # AF3239

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Primate - Chlorocebus aethiops (African Green Monkey), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Semaphorin 3E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3E
Thr25-Ser775 (Arg557Ala and Arg560Ala)
Accession # O15041

Specificity

Detects human Semaphorin 3E in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3F, and rhSemaphorin 3G is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Semaphorin 3E Antibody

Semaphorin 3E antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Semaphorin 3E in Mouse Brain.

Semaphorin 3E was detected in immersion fixed frozen sections of adult mouse brain using Goat Anti-Human Semaphorin 3E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3239) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cerebellum. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Semaphorin 3E in Mouse Lung.

Immunohistochemistry (paraffin-embedded) on Native Adult rat lung tissue. Sema33 stained green, DAPI (blue). Image from a verified customer review.
Detection of Semaphorin 3E by Immunohistochemistry

Detection of Semaphorin 3E by Immunohistochemistry

The impact of Sema3E‐Plexin D1 on the differentiation, proliferation, and migration of fibroblasts. A) Western blot analysis of Sema3E expression in primary human lung fibroblasts (PHLFs) following stimulation with different concentrations of TGF beta 1 for 48 h. B) Measurement of Sema3E concentration in culture media by ELISA. PHLFs were stimulated with TGF‐ beta 1, and Sema3E concentration was assessed after 48 h. C,D) Western blot and RT‐qPCR analysis of Fibronectin, Col1a1, and alpha ‐SMA protein and mRNA expression in Sema3E siRNA or Scrambled siRNA‐treated PHLFs following TGF‐ beta 1 induction. E,F) Western blot and RT‐qPCR analysis of Fibronectin, Col1a1, and alpha ‐SMA protein and mRNA expression in PlexinD1 siRNA or Scrambled siRNA‐treated PHLFs following TGF‐ beta 1 induction. G) Representative results for EdU staining in Sema3E siRNA or Scrambled siRNA‐treated PHLFs. H) Representative results for Transwell assay in Sema3E siRNA or Scrambled siRNA‐treated PHLFs. I) Representative results for EdU staining in Plexin D1 siRNA or Scrambled siRNA‐treated PHLFs. J) Representative results for Transwell assay in Plexin D1 siRNA or Scrambled siRNA‐treated PHLFs. Images were captured under original magnification ×200. Data are presented as the mean ± SEM of three independent experiments. Statistical analyses were performed using unpaired t‐tests and one‐way ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40112179), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Semaphorin 3E by Western Blot

Detection of Semaphorin 3E by Western Blot

Analysis of Sema3E and Plexin D1 levels in IPF Patients. A) ELISA analysis comparing Sema3E levels in the plasma of patients with IPF (n = 63) and control subjects (n = 24). B) Representative western blot analysis depicting Sema3E expression in plasma samples from IPF patients. C) Representative western blot analysis illustrating Sema3E and Plexin D1 expression in lung tissue samples from control subjects (n = 10) and IPF patients (n = 12). D–G) Correlation analysis between Sema3E levels and clinical parameters including (D) FVC%pred, (E) DLCO%pred, (F) TLC%pred, and (G) FEV1%pred in IPF patients (n = 32). H) Co‐immunostaining of Sema3E and alpha ‐SMA in lung sections from patients with IPF(n = 3) and control subjects(n = 3). I) Co‐immunostaining of Plexin D1 and alpha ‐SMA in lung sections from patients with IPF(n = 3) and control subjects(n = 3). Nuclei were stained blue with Diamidino‐2‐phenylindole dihydrochloride (DAPI), and images were captured under original magnification ×400. Data are presented as the mean ± SEM. Statistical analyses were performed using unpaired t‐tests and Pearson's correlation. *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significance levels. (FVC%pred: Forced Vital Capacity % predicted; TLC%pred: Total Lung Capacity % predicted; DLCO%pred: Diffusing Capacity of the Lung for Carbon Monoxide % predicted; FEV1%pred: Forced Expiratory Volume in 1 second % predicted.). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40112179), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Semaphorin 3E by Immunohistochemistry

Detection of Semaphorin 3E by Immunohistochemistry

Spatial and temporal patterns of expression for Sema3e and Plxnd1 in the mouse ovary around the time of ovulation. a) RT‐qPCR shows the mRNA levels of Sema3e and Plxnd1 in mouse ovarian GCs (n = 3), OSTs (n = 3), and whole ovaries (n = 3) before and after hCG treatment from superovulated immature mice. b) Representative images of IF staining of Sema3E in mouse ovaries (n = 3 for each time point) before and post‐hCG. The areas of staining for Sema3E were quantified by normalizing the Sema3E‐positive area to the Hoechst‐positive area of preovulatory follicles. Scale bar = 100 µm. c) Representative images of FISH of Plxnd1 (red) and Vegfr2 (green) mRNA in ovaries at 8 h (n = 4) and 24 h (n = 4) post‐hCG. Red arrowheads: Plxnd1+ cells. Green arrowheads: Vegfr2+ cells. Yellow arrowheads: Plxnd1+Vegfr2+ cells. White dashed lines indicate the boundary between the GCs/CL and OST. Scale bar = 50 µm. Quantitative data are presented as mean ± SD. One‐way ANOVA with Tukey's multiple comparisons test was used for statistical analysis in a (within the same cell/tissue types), b. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significance. GC: granulosa cell; OST: ovarian stromal tissue; OV: whole ovary; F: follicle; CL: corpus lute. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40391781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Semaphorin 3E by Western Blot

Detection of Semaphorin 3E by Western Blot

The impact of Sema3E‐Plexin D1 on the differentiation, proliferation, and migration of fibroblasts. A) Western blot analysis of Sema3E expression in primary human lung fibroblasts (PHLFs) following stimulation with different concentrations of TGF beta 1 for 48 h. B) Measurement of Sema3E concentration in culture media by ELISA. PHLFs were stimulated with TGF‐ beta 1, and Sema3E concentration was assessed after 48 h. C,D) Western blot and RT‐qPCR analysis of Fibronectin, Col1a1, and alpha ‐SMA protein and mRNA expression in Sema3E siRNA or Scrambled siRNA‐treated PHLFs following TGF‐ beta 1 induction. E,F) Western blot and RT‐qPCR analysis of Fibronectin, Col1a1, and alpha ‐SMA protein and mRNA expression in PlexinD1 siRNA or Scrambled siRNA‐treated PHLFs following TGF‐ beta 1 induction. G) Representative results for EdU staining in Sema3E siRNA or Scrambled siRNA‐treated PHLFs. H) Representative results for Transwell assay in Sema3E siRNA or Scrambled siRNA‐treated PHLFs. I) Representative results for EdU staining in Plexin D1 siRNA or Scrambled siRNA‐treated PHLFs. J) Representative results for Transwell assay in Plexin D1 siRNA or Scrambled siRNA‐treated PHLFs. Images were captured under original magnification ×200. Data are presented as the mean ± SEM of three independent experiments. Statistical analyses were performed using unpaired t‐tests and one‐way ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40112179), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Semaphorin 3E by Western Blot

Detection of Semaphorin 3E by Western Blot

Downregulation of Sema3E attenuates BLM‐induced pulmonary fibrosis. A,B) Histological analysis of lung fibrosis severity in mice following BLM induction. (A) Representative images of lung sections stained with H&E, Masson's trichrome, and Sirius red to assess fibrosis severity. (B) Bar graph showing the quantitative mean score of fibrosis severity. Samples include AAV9‐NC saline mice (n = 5), AAV9‐ShSema3E saline mice (n = 5), AAV9‐NC BLM mice (n = 5), and AAV9‐ShSema3E BLM mice (n = 5). C) Quantification of hydroxyproline contents in AAV9‐NC mice and AAV9‐ShSema3E mice after BLM challenge. D,E) Western blot and RT‐qPCR analysis of Fibronectin, Col1a1, and alpha ‐SMA protein and mRNA expression in lung homogenates from the mentioned mouse groups. F) Western blot analysis of ErbB2, P‐ErbB2, AKT, P‐AKT, ERK1/2, and P‐ERK1/2 expression in lung homogenates from the mentioned mouse groups. Samples include AAV9‐NC saline mice (n = 3), AAV9‐ShSema3E saline mice (n = 3), AAV9‐NC BLM mice (n = 3), and AAV9‐ShSema3E BLM mice (n = 3). Data are represented as the mean ± SEM. Statistical analyses were performed using one‐way ANOVA tests. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40112179), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Semaphorin 3E Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MDA-MB-453 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of adult mouse brain

Western Blot

0.1 µg/mL
Sample: Recombinant Human Semaphorin 3E (Catalog # 3239-S3)

Reviewed Applications

Read 1 review rated 4 using AF3239 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3E

Semaphorin 3E (Sema3E; previously SemaH) is one of six Class 3 (secreted) semaphorins which in the human share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels (2, 3). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure (4). C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (5, 6). Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins (7). Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity (7). Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (3, 8).

References

  1. Eichmann, A. et al. (2005) Genes Dev. 19:1013.
  2. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  3. Gu, C. et al. (2005) Science 307:265.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Christensen, C. et al. (1998) Cancer Res. 58:1238.
  6. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  7. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  8. Gitler, A. D. et al. (2004) Developmental Cell 7:107.

Alternate Names

Sema3E, SEMAH

Entrez Gene IDs

9723 (Human); 20349 (Mouse)

Gene Symbol

SEMA3E

UniProt

O15041

Additional Semaphorin 3E Products

Product Documents for Semaphorin 3E Antibody

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Product Specific Notices for Semaphorin 3E Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Semaphorin 3E Antibody

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  • Human/Mouse Semaphorin 3E Antibody
    Name: Anonymous
    Application: Immunohistochemistry (paraffin-embedded) on Native Adult rat lung tissue. Sema33 stained green, DAPI (blue).
    Sample Tested: Lung tissue
    Species: Rat
    Verified Customer | Posted 07/19/2025
    Semaphorin 3E Antibody AF3239
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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