Human/Mouse Semaphorin 6B Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human/Mouse Semaphorin 6B Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Semaphorin 6C is observed, 5% cross-reactivity with rhSemaphorin 6D is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, rhSemaphorin 6A, rhSemaphorin 7A, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, and rmSemaphorin 3F is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6B
Accession # Q9H3T3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 6B Fc Chimera (Catalog # 2094-S6)
Recombinant Mouse Semaphorin 6B
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6B

Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin-like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down‑regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8).

  1. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  2. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
  3. Correa, R. G. et al. (2001) Genomics 73:343.
  4. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
  5. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  6. Collet, P. et al. (2004) Genomics 83:1141.
  7. Murad, H. et al. (2006) Int. J. Oncol. 28:977.
  8. Suto, F. et al. (2005) J. Neurosci. 25:3628.
Entrez Gene IDs
10501 (Human); 20359 (Mouse)
Alternate Names
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353

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Citation for Human/Mouse Semaphorin 6B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Roles of semaphorin-6B and plexin-A2 in lamina-restricted projection of hippocampal mossy fibers.
    Authors: Tawarayama H, Yoshida Y, Suto F, Mitchell KJ, Fujisawa H
    J. Neurosci., 2010;30(20):7049-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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