Human/Mouse Semaphorin 6B Antibody
Human/Mouse Semaphorin 6B Antibody Summary
Accession # Q9H3T3
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 6B
Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin-like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down‑regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8).
- Neufeld, G. et al. (2005) Front. Biosci. 10:751.
- Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
- Correa, R. G. et al. (2001) Genomics 73:343.
- Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
- Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
- Collet, P. et al. (2004) Genomics 83:1141.
- Murad, H. et al. (2006) Int. J. Oncol. 28:977.
- Suto, F. et al. (2005) J. Neurosci. 25:3628.
Citation for Human/Mouse Semaphorin 6B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Roles of semaphorin-6B and plexin-A2 in lamina-restricted projection of hippocampal mossy fibers.
Authors: Tawarayama H, Yoshida Y, Suto F, Mitchell KJ, Fujisawa H
J. Neurosci., 2010;30(20):7049-60.
Sample Types: Tissue Homogenates
Applications: Western Blot
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