Detection of Human and Mouse Snapin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Snapin Monoclonal Antibody (Catalog # MAB7795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Snapin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Snapin (SNARE-associated protein) is an approximately 15 kDa, 136 amino acid vesicular phosphoprotein that contains a coiled-coil interacting domain. In neurons, it binds SNAP25 and RGS7 and regulates steps between synaptic vesicle docking and neurotransmitter release. In pancreatic beta cells, it mediates incretin-stimulated insulin secretion. In germ cells, it binds PUM2 and Nanos1 as a putative adaptor protein. Human Snapin shares 98% aa sequence identity with mouse and rat Snapin, while mouse and rat Snapin share 100% aa sequence identity with each other.
Entrez Gene IDs:
23557 (Human); 20615 (Mouse)
SNAP-25-binding protein; SNAP25BP; SNAPAP; SNAPAPSNARE-associated protein Snapin; SNAP-associated proteinSNARE associated protein snapin; Snapin; Synaptosomal-associated protein 25-binding protein
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