Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 329005
View all Sulfatase Modifying Factor 1/SUMF1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 1/SUMF1 isoform 1
Ser34-Asp374 (Ser63Asn)
Accession # Q8NBK3.3

Specificity

Detects recombinant human or mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SUMF2 or recombinant mouse SUMF2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Sulfatase Modifying Factor 1/SUMF1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human and mouse Sulfatase Modifying Factor 1/SUMF1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sulfatase Modifying Factor 1/SUMF1

Also known as C-alpha -formylglycine-generating enzyme (FGE), SUMF1 converts the active site Cys residue of sulfatases into formylglycine, which is essential for sulfatase activity. SUMF1 defect causes multiple sulfatase deficiency (MSD). The amino acid sequence of rhSUMF1 is 93%, 92% and 90% identical to that of canine, bovine, and mouse/rat.

Alternate Names

FGE1

Entrez Gene IDs

285362 (Human); 58911 (Mouse); 362409 (Rat)

Gene Symbol

SUMF1

UniProt

Q8NBK3.3

Additional Sulfatase Modifying Factor 1/SUMF1 Products

Product Documents for Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody

For research use only

Citations for Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody

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