Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VAMP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763

Specificity

Detects human and mouse VAMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VAMP-2 and less than 1% cross-reactivity with rhVAMP-5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse VAMP‑1 Antibody

Detection of Human/Mouse VAMP‑1 antibody by Western Blot.

Detection of Human/Mouse VAMP‑1 by Western Blot.

Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

VAMP‑1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr).

VAMP‑1 in Mouse Spinal Cord.

VAMP-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse VAMP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord

Western Blot

1 µg/mL
Sample: Human heart and brain tissue

Reviewed Applications

Read 2 reviews rated 5 using AF4828 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAMP-1

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is 118 amino acids (aa) in length. It is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1-96) and a 22 aa transmembrane domain (aa 97-118). There is one coiled-coil region between aa 33-93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Long Name

Vesicle-Associated Membrane Protein 1

Alternate Names

SYB1, Synaptobrevin-1, VAMP1

Entrez Gene IDs

6843 (Human); 22317 (Mouse); 25624 (Rat)

Gene Symbol

VAMP1

UniProt

P23763

Additional VAMP-1 Products

Product Documents for Human/Mouse VAMP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse VAMP‑1 Antibody

For research use only

Citations for Human/Mouse VAMP‑1 Antibody

Customer Reviews for Human/Mouse VAMP‑1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human/Mouse VAMP-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 07/02/2022
    Used at 1:500 in 5% non-fat milk overnight at 4 degree
    Human/Mouse VAMP‑1 Antibody AF4828
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23206873
    Species: Rat
    Verified Customer | Posted 01/08/2015

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