Human/Mouse VAMP-1 Antibody Summary
Accession # P23763
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
VAMP‑1 in Mouse Spinal Cord. VAMP‑1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human/Mouse VAMP‑1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP‑1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is 118 amino acids (aa) in length. It is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1-96) and a 22 aa transmembrane domain (aa 97-118). There is one coiled-coil region between aa 33-93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.
Citation for Human/Mouse VAMP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
Mol Brain, 2012;5(0):22.
Sample Types: Tissue Homogenates
Applications: Western Blot
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