Human/Mouse VAMP-1 Antibody Summary
Met1-Lys96
Accession # P23763
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse VAMP‑1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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VAMP‑1 in Mouse Spinal Cord. VAMP-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAMP-1
VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is 118 amino acids (aa) in length. It is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1-96) and a 22 aa transmembrane domain (aa 97-118). There is one coiled-coil region between aa 33-93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.
Product Datasheets
Citations for Human/Mouse VAMP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Dependence receptor involvement in subtilisin-induced long-term depression and in long-term potentiation
Neuroscience, 2016-08-31;336(0):49-62.
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Mesial Temporal Lobe Epilepsy (MTLE) Drug-Refractoriness Is Associated With P2X7 Receptors Overexpression in the Human Hippocampus and Temporal Neocortex and May Be Predicted by Low Circulating Levels of miR-22
Authors: B Guerra Lea, A Barros-Bar, F Ferreirinh, J Chaves, R Rangel, A Santos, C Carvalho, R Martins-Fe, R Samões, J Freitas, J Lopes, J Ramalheira, MG Lobo, A Martins da, PP Costa, P Correia-de
Frontiers in Cellular Neuroscience, 2022-07-07;16(0):910662.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Prenatal inhibition of the kynurenine pathway leads to structural changes in the hippocampus of adult rat offspring
Authors: Omari S. Khalil, Mazura Pisar, Caroline M. Forrest, Maria C. J. Vincenten, L. Gail Darlington, Trevor W. Stone
European Journal of Neuroscience
Species: Rat
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway
Authors: C.M. Forrest, O.S. Khalil, M. Pisar, K. McNair, E. Kornisiuk, M. Snitcofsky et al.
Neuroscience
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Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
Mol Brain, 2012-06-09;5(0):22.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation
Authors: Dong Z, Chen W, Chen C et al.
Neuron
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Used at 1:500 in 5% non-fat milk overnight at 4 degree
