Human/Mouse VAMP-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse VAMP‑1 by Western Blot.
2 Images
Product Details
Citations (2)
Supplemental Products

Human/Mouse VAMP-1 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse VAMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VAMP-2 and less than 1% cross-reactivity with rhVAMP-5 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human VAMP-1
Accession # P23763
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse VAMP‑1 antibody by Western Blot. View Larger

Detection of Human/Mouse VAMP‑1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry VAMP‑1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

VAMP‑1 in Mouse Spinal Cord. VAMP-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-1

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is 118 amino acids (aa) in length. It is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1-96) and a 22 aa transmembrane domain (aa 97-118). There is one coiled-coil region between aa 33-93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Long Name
Vesicle-Associated Membrane Protein 1
Entrez Gene IDs
6843 (Human); 22317 (Mouse); 25624 (Rat)
Alternate Names
DKFZp686H12131; SYB1; SYB1vesicle-associated membrane protein 1; synaptobrevin 1; Synaptobrevin-1; VAMP1; VAMP-1; VAMP-1synaptobrevin-1; vesicle-associated membrane protein 1 (synaptobrevin 1)

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Citations for Human/Mouse VAMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mesial Temporal Lobe Epilepsy (MTLE) Drug-Refractoriness Is Associated With P2X7 Receptors Overexpression in the Human Hippocampus and Temporal Neocortex and May Be Predicted by Low Circulating Levels of miR-22
    Authors: B Guerra Lea, A Barros-Bar, F Ferreirinh, J Chaves, R Rangel, A Santos, C Carvalho, R Martins-Fe, R Samões, J Freitas, J Lopes, J Ramalheira, MG Lobo, A Martins da, PP Costa, P Correia-de
    Frontiers in Cellular Neuroscience, 2022;16(0):910662.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012;5(0):22.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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