Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for WDR5 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
WDR5 in Human B-Cell Lymphoma.
WDR5 was detected in immersion fixed paraffin-embedded sections of human B-cell lymphoma using Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a nuclear, 36‑40 kDa monomeric member of the WD family of repeat proteins. It is known to be expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is 334 amino acids (aa) in length. It contains seven WD domains (aa 43‑333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.
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