Human/Mouse WDR5 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse WDR5 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human WDR5
    Met1-Lys120
    Accession # P61964
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human WDR5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for WDR5 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry
WDR5 in Human B-Cell Lymphoma. WDR5 was detected in immersion fixed paraffin-embedded sections of human B-cell lymphoma using Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WDR5
WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a nuclear, 36‑40 kDa monomeric member of the WD family of repeat proteins. It is known to be expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is 334 amino acids (aa) in length. It contains seven WD domains (aa 43‑333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.
  • Long Name:
    WD Repeat-containing Protein 5
  • Entrez Gene IDs:
    11091 (Human); 140858 (Mouse); 362093 (Rat)
  • Alternate Names:
    BIG3; BIG-3; BMP2-induced 3-kb gene protein; SWD3; SWD3, Set1c WD40 repeat protein, homolog; WD repeat domain 5; WD repeat-containing protein 5; WDR5; WD-repeat protein 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. PI3K/AKT-mediated upregulation of WDR5 promotes colorectal cancer metastasis by directly targeting ZNF407
    Authors: X Tan, S Chen, J Wu, J Lin, C Pan, X Ying, Z Pan, L Qiu, R Liu, R Geng, W Huang
    Cell Death Dis, 2017;8(3):e2686.
  2. GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program.
    Authors: Hopkin, Amelia S, Gordon, William, Klein, Rachel H, Espitia, Francisc, Daily, Kenneth, Zeller, Michael, Baldi, Pierre, Andersen, Bogi
    PLoS Genet, 2012;8(7):e1002829.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis.
    Authors: Liu L, Yin Y, Li Y
    Cell Res., 2012;22(8):1258-69.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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