Human MS4A3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MS4A3 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 489433
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human MS4A3
    Accession # NP_006129
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MS4A3 in Human PBMC by Flow Cytometry. Human peripheral blood monocytes were stained with Human MS4A3 Monoclonal Antibody (Catalog # MAB6247, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0103B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MS4A3

MS4A3, also known as HTm4, is an intracytoplasmic membrane protein that is a member of the MS4A family of four-transmembrane proteins that also includes CD20 and Fc epsilon RI beta.  It is a cell cycle regulator expressed in the perinuclear area of human hematopoietic cells. The C-terminal region of MS4A3 interacts with cdc-associated phosphatase (KAP), enhancing its activity on cyclin-dependent kinase 2 (cdk2) and facilitating G(0)/G(1) arrest. The combined non-membrane segments of the 214 amino acid (aa), 25 kDa human MS4A3 show 53% and 52% aa identity with corresponding regions of mouse and rat MS4A3, respectively.

  • Long Name:
    Membrane-spanning 4-domains, Subfamily A, Member 3 [Hematopoietic Cell-specific]
  • Entrez Gene IDs:
    932 (Human); 170813 (Mouse); 293753 (Rat)
  • Alternate Names:
    CD20 antigen homolog; CD20 antigen-like protein; CD20L; CD20LIgE receptor beta subunit; hematopoietic cell 4 transmembrane protein; Hematopoietic-specific transmembrane protein 4; HTM4; HTM4IgE receptor beta chain; membrane-spanning 4-domains subfamily A member 3; membrane-spanning 4-domains, subfamily A, member 3 (hematopoieticcell-specific); MS4A3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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