MS4A3, also known as HTm4, is an intracytoplasmic membrane protein that is a member of the MS4A family of four-transmembrane proteins that also includes CD20 and Fc epsilon RI beta. It is a cell cycle regulator expressed in the perinuclear area of human hematopoietic cells. The C-terminal region of MS4A3 interacts with cdc-associated phosphatase (KAP), enhancing its activity on cyclin-dependent kinase 2 (cdk2) and facilitating G(0)/G(1) arrest. The combined non-membrane segments of the 214 amino acid (aa), 25 kDa human MS4A3 show 53% and 52% aa identity with corresponding regions of mouse and rat MS4A3, respectively.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 489433
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human MS4A3
Accession # NP_006129
Accession # NP_006129
Specificity
Detects human MS4A3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human MS4A3 Antibody
Detection of MS4A3 in Human PBMC by Flow Cytometry.
Human peripheral blood monocytes were stained with Human MS4A3 Monoclonal Antibody (Catalog # MAB6247, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0103B).
Applications for Human MS4A3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MS4A3
Long Name
Membrane-spanning 4-domains, Subfamily A, Member 3 [Hematopoietic Cell-specific]
Alternate Names
CD20L, HTM4
Gene Symbol
MS4A3
UniProt
Additional MS4A3 Products
Product Documents for Human MS4A3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MS4A3 Antibody
For research use only
Related Research Areas
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