MS4A4A, also called CD20-like 1, is a 23 kDa (predicted) member of the MS4A family of four-transmembrane proteins that includes CD20 (MS4A1) and Fce RIb (MS4A2). MS4A4 cDNA is detected in hematopoietic cell lines, with highest expression in myeloid and B cell lines. Expressed sequence tags are detected in multiple organs and tissues. Human MS4A4A shares ~60% amino acid sequence identity with mouse and rat MS4A4A, if sequence gaps at the cytoplasmic N and C termini are disregarded. One potential isoform lacks aa 130-182, which includes the 3rd transmembrane segment and the first of two extracellular regions.
Human MS4A4A Antibody
R&D Systems | Catalog # MAB7797
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 818112
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Product Specifications
Immunogen
Mouse myeloma cell line NS0 transfected with human MS4A4A
Accession # Q96JQ5
Accession # Q96JQ5
Specificity
Detects human MS4A4A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MS4A4A Antibody
Detection of MS4A4A in HEK293 Human Cell Line Co-transfected with MS4A4A and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line co-transfected with human MS4A4A and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human MS4A4A Monoclonal Antibody (Catalog # MAB7797) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).Applications for Human MS4A4A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µL/106 cells
Sample: Human MS4A4A and eGFP co-transfectants
Sample: Human MS4A4A and eGFP co-transfectants
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MS4A4A
Long Name
Membrane-spanning 4-domains, Subfamily A, Member 4A
Alternate Names
4SPAN1, CD20L1, MS4A4, MS4A7
Gene Symbol
MS4A4A
UniProt
Additional MS4A4A Products
Product Documents for Human MS4A4A Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MS4A4A Antibody
For research use only
Citations for Human MS4A4A Antibody
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