Human MSP/MST1 Antibody
Human MSP/MST1 Antibody Summary
Gln19-Gly711
Accession # AAA59872
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody. Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF352). The ND50 is typically 0.05-0.2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSP/MST1
Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).
- Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
- Han, S. et al. (1991) Biochemistry 30:9768.
- Muraoka, R.S. et al. (1999) Endocrinology 140:187.
- Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
- Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
- Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
- Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
- Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
- Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
- Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
- Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
- Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
Product Datasheets
Citations for Human MSP/MST1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
Authors: Rampino T, Collesi C, Gregorini M
J. Am. Soc. Nephrol., 2002;13(3):649-57.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
Sci Rep, 0;7(0):42725.
Species: Mouse
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation
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Reviews for Human MSP/MST1 Antibody
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We used this antibody for developing a sandwich ELISA in combination with mAb (MAB352) and protein (352-MS). This combination worked well and detected human MSP in serum samples efficiently.
