Human MSP/MST1 Antibody

Catalog # Availability Size / Price Qty
AF352
AF352-SP
Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody.
4 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human MSP/MST1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human HGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP/MST1
Gln19-Gly711
Accession # AAA59872
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MSP (Catalog # 352-MS)
Neutralization
Measured by its ability to neutralize MSP-induced chemotaxis in mouse peritoneal resident macrophages. Ming-Hai, W. et al. (1994) J. Biol. Chem. 269:3436‑3440. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 50 ng/mL Recombinant Human MSP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody. View Larger

Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody. Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF352). The ND50 is typically 0.05-0.2 µg/mL.

Flow Cytometry Detection of Mouse MSP/MST1 by Flow Cytometry View Larger

Detection of Mouse MSP/MST1 by Flow Cytometry TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunoprecipitation Detection of Mouse MSP/MST1 by Immunoprecipitation View Larger

Detection of Mouse MSP/MST1 by Immunoprecipitation TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunoprecipitation Detection of Mouse MSP/MST1 by Immunoprecipitation View Larger

Detection of Mouse MSP/MST1 by Immunoprecipitation TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

References
  1. Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
  2. Han, S. et al. (1991) Biochemistry 30:9768.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Entrez Gene IDs
4485 (Human); 15235 (Mouse); 24566 (Rat)
Alternate Names
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2

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Citations for Human MSP/MST1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Comprehensive analysis of receptor tyrosine kinase activation in human melanomas reveals autocrine signaling through IGF-1R
    Authors: Kerrington R. Molhoek, Amber L. Shada, Mark Smolkin, Sudhir Chowbina, Jason Papin, David L. Brautigan et al.
    Melanoma Research
  2. Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
    Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
    Sci Rep, 2017-02-17;7(0):42725.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Co-Immunoprecipitation, Flow Cytometry
  3. Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
    Authors: Rampino T, Collesi C, Gregorini M
    J. Am. Soc. Nephrol., 2002-03-01;13(3):649-57.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization, Western Blot

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Human MSP/MST1 Antibody
By Anonymous on 09/23/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for developing a sandwich ELISA in combination with mAb (MAB352) and protein (352-MS). This combination worked well and detected human MSP in serum samples efficiently.