Musashi‑2 in Rat Cortical Stem Cells. Musashi‑2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Musashi‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3255) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Musashi-2 belongs to the evolutionarily conserved Musashi family of RNA
binding proteins which are involved in the translational control of
their target mRNAs. Musashi-2 has two tandem RNA-recognition domains
(RRM-1 and RRM-2) that have the highly conserved RNP
(ribonucleoprotein) motifs. By alternative splicing, at least three
Musashi-2 isoforms exist. In mammalian nervous system, Musashi-1 and –2
are selectively expressed in neural progenitor cells and play important
roles in maintenance of the stem cell fate. Human Musashi-2 shares 95%
amino acid sequence homology with mouse Musashi-2 and 80% amino acid
sequence identity with human Musashi-1. Translocations resulting in the
formation of Musashi-2/HOXA9 fusion protein is associated with
progression of chronic myelogenous leukemia to the accelerated phase
and blast crisis.
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