Human NAALADase-2/NAALAD2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NAALADase-2/NAALAD2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NAALADase-2 is observed and no cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like 2, or rhNAALADase-like 1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 756030
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-2/NAALAD2
    Lys32-Leu740
    Accession # Q9Y3Q0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NAALADase-2/NAALAD2 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
NAALADase-2/NAALAD2 in Human Testis. NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/
NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NAALADase-2/NAALAD2
N-acetylated-alpha-linked acidic dipeptidase 2 (NAALADase-2), also known as glutamate carboxypeptidase 3 (GCP3), is a 90-100 kDa type 2 transmembrane protein in the M28 peptidase family. It hydrolyzes beta-citrylglutamate (BCG) and N-acetyl-aspartylglutamate (NAAG) which are abundant dipeptides found in brain and testis. Mature human NAALADase-2 consists of a 7 amino acid (aa) cytoplasmic domain, a 24 aa transmembrane segment, and a 709 aa extracellular region (aa 32-740) that contains the peptidase domain. Over aa 32-740, human NAALADase-2 shares 89% aa sequence identity with mouse NAALADase-2.
  • Long Name:
    N-Acetylated Alpha-Linked Acidic Dipeptidase 2
  • Entrez Gene IDs:
    10003 (Human); 72560 (Mouse); 300384 (Rat)
  • Alternate Names:
    GCPIII; Glutamate Carboxypeptidase III; GPCIII; MGC116996; MGC26353; NAADALASE2; NAALAD2; NAALADase II; NAALADase2; NAALADase-2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase II; N-acetylated-alpha-linked acidic dipeptidase 2; N-acetylated-alpha-linked acidic dipeptidase II
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