Human NAALADase-2/NAALAD2 Antibody

Catalog # Availability Size / Price Qty
MAB7658
MAB7658-SP
Detection of Human NAALADase-2/NAALAD2 by Western Blot.
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Human NAALADase-2/NAALAD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NAALADase-2/NAALAD2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NAALADase-2 is observed and no cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like 2, or rhNAALADase-like 1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 756030
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-2/NAALAD2
Lys32-Leu740
Accession # Q9Y3Q0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human NAALADase-2/NAALAD2 antibody by Western Blot. View Larger

Detection of Human NAALADase-2/NAALAD2 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry NAALADase-2/NAALAD2 antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

NAALADase-2/NAALAD2 in Human Testis. NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/ NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAALADase-2/NAALAD2

N-acetylated-alpha-linked acidic dipeptidase 2 (NAALADase-2), also known as glutamate carboxypeptidase 3 (GCP3), is a 90-100 kDa type 2 transmembrane protein in the M28 peptidase family. It hydrolyzes beta-citrylglutamate (BCG) and N-acetyl-aspartylglutamate (NAAG) which are abundant dipeptides found in brain and testis. Mature human NAALADase-2 consists of a 7 amino acid (aa) cytoplasmic domain, a 24 aa transmembrane segment, and a 709 aa extracellular region (aa 32-740) that contains the peptidase domain. Over aa 32-740, human NAALADase-2 shares 89% aa sequence identity with mouse NAALADase-2.

Long Name
N-Acetylated Alpha-Linked Acidic Dipeptidase 2
Entrez Gene IDs
10003 (Human); 72560 (Mouse); 300384 (Rat)
Alternate Names
GCPIII; Glutamate Carboxypeptidase III; GPCIII; MGC116996; MGC26353; NAADALASE2; NAALAD2; NAALADase II; NAALADase2; NAALADase-2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase II; N-acetylated-alpha-linked acidic dipeptidase 2; N-acetylated-alpha-linked acidic dipeptidase II

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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