Human NAALADase-like 2/NAALADL2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NAALADase-like 2/NAALADL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
    5% cross‑reactivity with recombinant mouse NAALADase 2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NAALADase-like 2/NAALADL2
    Ser152-Asn795 (Met194Thr)
    Accession # CAE75743
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NAALADase-like 2/NAALADL2
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human NAALADase-like 2/
    NAALADL2, see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed PC-3 human prostate cancer cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NAALADase‑like 2/
NAALADL2 in LnCAP Human Cell Line by Flow Cytometry.

LnCAP human prostate cancer cell line was stained with Goat Anti-Human NAALADase‑like 2/NAALADL2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4665, filled histogram) or control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoe­rythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively.

  • Long Name:
    N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2
  • Entrez Gene IDs:
    254827 (Human); 635702 (Mouse); 499583 (Rat)
  • Alternate Names:
    inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2; NAALADase L2; NAALADase like 2; NAALADase-like 2; NAALADL2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase-like 2
Related Research Areas
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