Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 756465
View all NELL1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human human NELL1
Arg17-Asn810
Accession # Q92832

Specificity

Detects human NELL1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse NELL1 or recombinant human NELL2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NELL1 Antibody

Detection of Human NELL1 antibody by Western Blot.

Detection of Human NELL1 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NELL1 Monoclonal Antibody (Catalog # MAB5487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NELL1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NELL1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB5487 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NELL1

NELL1 (neural EGF-like like protein 1) is an 89 kDa (predicted) member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists as a phosphoglycoprotein that can add as much as 50 kDa to the calculated MW. NELL1 has restricted expression, being limited to pre-B cells and osteoblasts, where it apparently promotes osteoblast maturation and bone formation. In tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and retained intracellularly where it is phosphorylated by PKC. The human NELL1 precursor is 810 amino acids (aa) in length. It contains a 16 aa signal sequence plus a 794 aa mature region. The mature region possesses an N-terminal TSP domain (aa 81-230), two VWFC domains (aa 271-390), six consecutive EGF-like domains (aa 391-631), and three additional C-terminal VWFC domains (aa 632-807). Secreted NELL1 forms a 400-420 kDa noncovalent homotrimer. Over aa 17-810, human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternate splicing generates an additional isoform of human NELL1 that lacks the fifth EGF-like domain.

Long Name

NEL-like 1

Alternate Names

IDH3GL

Entrez Gene IDs

4745 (Human); 338352 (Mouse)

Gene Symbol

NELL1

UniProt

Q92832

Additional NELL1 Products

Product Documents for Human NELL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NELL1 Antibody

For research use only

Customer Reviews for Human NELL1 Antibody (1)

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  • Human NELL1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult kidney
    Species: Human
    Verified Customer | Posted 05/15/2023
    Detection of NELL1 in Human Kidney Lysate 0.4 mg/ml Simple Western 12-230 kDa Separation Module Blocking: Antibody Diluent 2, Primary antibody: MAB5487 Antibody concentration 1:100 Secondary Antibody: Mouse Secondary Antibody 042-205 Band at 117 kDa
    Human NELL1 Antibody MAB5487

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