Detection of Human NELL1 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NELL1 Monoclonal Antibody (Catalog # MAB5487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NELL1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(neural EGF-like like protein 1) is an 89 kDa
(predicted) member of the EGF-like domain containing family, Laminin
G/N-TSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists
as a phosphoglycoprotein that can add as much as 50 kDa to the calculated MW.
NELL1 has restricted expression, being limited to pre-B cells and osteoblasts,
where it apparently promotes osteoblast maturation and bone formation. In
tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and
retained intracellularly where it is phosphorylated by PKC. The human NELL1
precursor is 810 amino acids (aa) in length. It contains a 16 aa signal
sequence plus a 794 aa mature region. The mature region possesses an N-terminal
TSP domain (aa 81-230), two VWFC domains (aa 271-390), six consecutive EGF-like
domains (aa 391-631), and three additional C-terminal VWFC domains (aa
632-807). Secreted NELL1 forms a 400-420 kDa noncovalent homotrimer. Over aa
17-810, human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternate
splicing generates an additional isoform of human NELL1 that lacks the fifth
Entrez Gene IDs:
4745 (Human); 338352 (Mouse)
FLJ45906; IDH3GL; nel (chicken)-like 1; NELL1; NEL-like 1 (chicken); NEL-like protein 1; Nel-related protein 1; neural epidermal growth factor-like 1; NRP1; protein kinase C-binding protein NELL1
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