Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1037720
View all Nephrin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived human Nephrin
Gln23-Thr1029
Accession # O60500

Specificity

Detects human Nephrin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Nephrin Antibody

Detection of Human Nephrin by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line and human kidney (medulla). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nephrin Monoclonal Antibody (Catalog # MAB42692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nephrin at approximately 134 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Nephrin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line and human kidney (medulla)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nephrin

Nephrin (or renal glomerulus-specific cell adhesion receptor; also NPHS1) is a 185 - 200 kDa member of the Ig superfamily. It is expressed on glomerular podocyte membranes, associates with same-cell Neph1, and forms homomeric zipper-like complexes with Nephrin on adjacent podocytes. Mature human Nephrin is a type I transmembrane glycoprotein 1219 amino acids (aa) in length. It contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential, soluble splice form is known where aa’s 1056 - 1095 are deleted, eliminating the transmembrane region. Over aa 23 - 1029, human Nephrin shares 84% and 89% aa identity with mouse and dog Nephrin, respectively.

Alternate Names

CNF, NPHS1

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Gene Symbol

NPHS1

UniProt

O60500

Additional Nephrin Products

Product Documents for Human Nephrin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Nephrin Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human Nephrin Antibody

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