Human Neudesin Antibody

Catalog # Availability Size / Price Qty
MAB6714
MAB6714-SP
Detection of Human Neudesin by Western Blot.
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Product Details
Citations (1)
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Human Neudesin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neudesin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 737130
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neudesin
Gly32-Phe172
Accession # Q9UMX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neudesin antibody by Western Blot. View Larger

Detection of Human Neudesin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, IMR-32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neudesin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neudesin antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Neudesin in Human Brain. Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neudesin

Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human Neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human Neudesin shares 97% aa identity with mature mouse Neudesin

Entrez Gene IDs
29937 (Human); 66208 (Mouse); 289380 (Rat)
Alternate Names
Cell immortalization-related protein 2; CIR2; CIR2cell growth-inhibiting protein 47; NENF; Neudesin; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF protein; SPUF; SPUFneudesin

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Citation for Human Neudesin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression
    Authors: Kami?ska, J;Koper-Lenkiewicz, OM;Ponikwicka-Tyszko, D;Lebiedzi?ska, W;Palak, E;Sztachelska, M;Bernaczyk, P;Dorf, J;Guzi?ska-Ustymowicz, K;Zar?ba, K;Wo?czy?ski, S;Rahman, NA;Dymicka-Piekarska, V;
    Cancers
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC

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