Human Neurogenin-3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Neurogenin-1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 258602
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Neurogenin-3
    Met1-Leu214
    Accession # Q9Y4Z2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Neurogenin‑3 by Western Blot. Western blot shows lysates of human heart tissue, human placenta tissue, human prostate tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Neurogenin‑3 Monoclonal Antibody (Catalog # MAB3444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neurogenin‑3 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurogenin-3
Neurogenin-3 (NGN3) is a 23 kDa developmentally expressed nuclear protein of the basic helix-loop-helix (bHLH) family, class B subfamily. bHLH proteins are transcription factors that form dimers to bind DNA. Neurogenin-3 expression is required for all endocrine lineage differentiation in the pancreas, including formation of islet beta  cells. Neurogenin-3 is also neurogenic, controlling the number of dendrites developed by hippocampal neurons. Human Neurogenin-3 shows 76% and 77% amino acid identity with mouse and rat Neurogenin-3, respectively.
  • Entrez Gene IDs:
    50674 (Human); 11925 (Mouse); 60329 (Rat)
  • Alternate Names:
    Atoh5; BHLHA7; bHLHa7NGN-3; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; neurogenin 3; Neurogenin3; Neurogenin-3; NGN3; Protein atonal homolog 5; Relax
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 24  
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Recombinant Mouse IgG2A Fc

BA 4460-MG 1
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Mouse IgG2A Isotype Control

Ctrl MAB0031 5  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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