Human Neurturin Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Neurturin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Neurturin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Neurturin
    Ala96-Val197
    Accession # Q99748
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Neurturin (Catalog # 387-NE)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (cerebellum) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
  • Neutralization
    Measured by its ability to neutralize the effect of Neurturin on dorsal root ganglion neurons from E10 chick embryos. Davies, A. M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R. A. (eds): John Willey and Sons, Ltd. 95. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 6 ng/mL Recombinant Human Neurturin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Neuron Survival in Response to Neurturin and Neutralization by Human Neurturin Antibody. Recombinant Human Neurturin (Catalog # 387-NE) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human Neurturin (6 ng/mL) is neutralized (green line) by increasing concentrations of Human Neurturin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF387). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurturin

Neurturin (NTN) is a member of the GDNF family of ligands which also includes glial cell-derived neurotrophic factor (GDNF), persephin, and artemin. GDNF family proteins are distant members of the TGF-beta superfamily and contain a conserved seven cysteine motif found in the entire family. Human NTN encodes a 197 amino acid (aa) preproprotein with a 19 aa residue putative signal peptide and a 76 aa pro region. Proteolytic cleavage of the pro-protein occurs at an RXXR consensus sequence. The native protein is a disulfide-linked homodimer with a calculated monomeric mass of about 12.5 kDa. The amino acid sequence of human NTN shares 91% identity to mouse NTN. NTN also shares about 42% similarity with GDNF. The bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 - GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase). NTN prefers to bind GFR alpha 2 but can also bind GFR alpha 1. NTN can promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. The wide expression of NTN in both neuronal and non-neuronal tissues suggests that NTN may regulate the development and maintenance of the central and peripheral nervous systems and other systems.

  • References:
    1. Kotzbauer, P. et al. (1996) Nature 384:467.
    2. Baloh, R.H. et al. (1997) Neuron 18:793.
    3. Baloh, R.H. et al. (1998) Neuron 21:1291.
  • Entrez Gene IDs:
    4902 (Human); 18188 (Mouse)
  • Alternate Names:
    Neurturin; NRTN; NTN
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016;535(7612):440-3.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Blocking
  2. Neurturin gene therapy improves motor function and prevents death of striatal neurons in a 3-nitropropionic acid rat model of Huntington's disease.
    Authors: Ramaswamy S, McBride JL, Herzog CD, Brandon E, Gasmi M, Bartus RT, Kordower JH
    Neurobiol. Dis., 2007;26(2):375-84.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Delivery of neurturin by AAV2 (CERE-120)-mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP-treated monkeys.
    Authors: Kordower JH, Herzog CD, Dass B, Bakay RA, Stansell J, Gasmi M, Bartus RT
    Ann. Neurol., 2006;60(6):706-15.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
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