Human NF2/Merlin Antibody

Catalog # Availability Size / Price Qty
MAB5616
MAB5616-SP
Human NF2/Merlin Antibody in Data
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Human NF2/Merlin Antibody Summary

Species Reactivity
Human
Specificity
Detects human NF2/Merlin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NF1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 534428
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NF2/Merlin
Met363-Lys578
Accession # P35240
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NF2/Merlin antibody by Western Blot. View Larger

Detection of Human NF2/Merlin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human NF2/Merlin Monoclonal Antibody (Catalog # MAB5616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF2/Merlin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF2/Merlin

NF2 (Neurofibromin-2; also Merlin) is a 70‑75 kDa tumor suppressor protein belonging to the ERM (ezrin, radixin, moesin) family of proteins. It participates in contact‑mediated cell adhesion, thereby blocking cell proliferation and migration. Human NF2 is 595 amino acids (aa) in length. It contains three general regions. There is an N‑terminal FERM domain (aa 1‑302), an alpha -helical rod central region (aa 303‑478), and a unique carboxy-terminal domain (aa 479‑595). Confluence requires an absence of phosphorylation at Ser516 and Ser10. There are multiple potential splice variants. Four short forms less than 260 aa in length lack the aa range used for antigen immunization. There are at least eight additional isoforms that basically show an alternate start site coupled to an 11 aa substitution for aa 579‑595. Lack of NF2 is associated with neurofibromatosis type II.

Long Name
Neurofibromin 2
Entrez Gene IDs
4771 (Human); 18016 (Mouse); 25744 (Rat)
Alternate Names
ACN; BANF; Merlin; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); neurofibromin-2; NF2; SCH; Schwannomerlin; Schwannomin

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NF2/Merlin Antibody

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Human NF2/Merlin Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human