Detection of Human NGL‑3/LRRC4B by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGL‑3/LRRC4B Monoclonal Antibody (Catalog # MAB69191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGL‑3/LRRC4B at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NGL-3 (netrin-G ligand-3), also called LRRC4B (Leu-rich repeat/LRR-containing glycoprotein 4B), is a 72 kDa (predicted) post-synaptic adhesion molecule of the LRR protein family. Mature human LRRC4B is a 678 amino acid (aa) type I transmembrane protein with a 541 aa extracellular region (aa 36-576) containing nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. Over the region used as an immunogen, human LRRC4B shares 97% aa sequence identity with mouse and rat LRRC4B. LRRC4B also shares ~55% aa identity with LRRC4C/NGL-1 and LRRC4/NGL-2, but each recognizes different ligands. LRRC4B recognition of the receptor tyrosine phosphatases LAR/PTPRF, PTPR-d and PTPR-s induces excitatory synapse formation.
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