Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 333413
View all NgR2/NgRH1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NgR2/NgRH1
Ala28-Leu401
Accession # Q86UN3

Specificity

Detects human NgR2/NgRH1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) NgR3 or rhNgR is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human NgR2/NgRH1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human NgR2/NgRH1

Reviewed Applications

Read 2 reviews rated 3.5 using MAB2776 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NgR2/NgRH1

NgR2, also known as reticulon 4 receptor-like 2 and NgRH1, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- as well as the C-terminus. Membrane-anchored NgR2 can be solubilized through the action of phospholipase or an unidentified MT-MMP to generate a 46 kDa soluble receptor. Mature human NgR2 shares 48% aa sequence identity with human NgR3. It also shares 96% aa sequence identity with mouse and rat mature NgR2.

Long Name

Nogo-66 Receptor-related Protein 2/Nogo-66 Receptor Homolog 1/Reticulon-4 Receptor-like 2

Alternate Names

NgRH1, RTN4RL2

Entrez Gene IDs

349667 (Human); 269295 (Mouse)

Gene Symbol

RTN4RL2

UniProt

Q86UN3

Additional NgR2/NgRH1 Products

Product Documents for Human NgR2/NgRH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NgR2/NgRH1 Antibody

For research use only

Customer Reviews for Human NgR2/NgRH1 Antibody (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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3 Stars
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1 Stars
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  • Human NgR2/NgRH1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human NgR2/NgRH1 Antibody MAB2776
  • Human NgR2/NgRH1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019

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