Human NK1R Antibody

Catalog # Availability Size / Price Qty
Detection of Human NK1R by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human NK1R Antibody Summary

Species Reactivity
Detects human NK1R in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 694549
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human NK1R
Accession # P25103
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NK1R antibody by Western Blot. View Larger

Detection of Human NK1R by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human NK1R Monoclonal Antibody (Catalog # MAB6687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NK1R at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NK1R

NK1R (Neurokinin-1 receptor), gene name TACR1 (tachykinin receptor-1), is an ~80 kDa 7-transmembrane glycoprotein receptor for the proinflammatory tachykinin neuropeptide, substance P. NK1R is constitutively or inducibly expressed on a wide variety of cells, including monocytes, macrophages, microglia, lymphocytes, neutrophils, mast cells, and neurons. The 407 amino acid (aa) human NK1R contains 89 extracellular aa over multiple segments which collectively share 92% aa identity with corresponding regions of mouse and rat NK1R. A ~50 kDa short isoform that ends at aa 311 lacks almost all of the C-terminal cytoplasmic signaling region. It is the only form expressed in human monocytes and undifferentiated THP-1 cells, and its function appears to differ from the long isoform.

Long Name
Neurokinin-1 Receptor
Entrez Gene IDs
6869 (Human); 21336 (Mouse); 24807 (Rat)
Alternate Names
NK1R; NK-1R; NK1Rneurokinin 1 receptor; NKIR; SPR; SPRsubstance-P receptor; TAC1R; TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor); tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor); tachykinin receptor 1NK-1 receptor; TACR1

Product Datasheets

You must select a language.


Citation for Human NK1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ.
    Authors: Sloniecka M, Le Roux S, Boman P, Bystrom B, Zhou Q, Danielson P
    PLoS ONE, 2015;10(7):e0134157.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NK1R Antibody

There are currently no reviews for this product. Be the first to review Human NK1R Antibody and earn rewards!

Have you used Human NK1R Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review