Human NKG2H Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NKG2H in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 633810
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human NKG2H synthetic peptide
    HQIKFYICSNRND
    Accession # Q07444
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NKG2H by Western Blot. Western blot shows lysates of human CD56+ NK cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NKG2H Monoclonal Antibody (Catalog # MAB6549) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKG2H at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2H

Human NKG2H (NK cell Group 2 isoform H; also NK cell receptor H) is a 38 kDa member of the C-type lectin-like domain superfamily of molecules. It represents an alternative splice form of the NKG2E/KLRC3 gene, one of three related genes on human Ch12 that give rise to five isoform variants (NKG2A & B, C, E & H). NKG2H is present on both NK and CD8+ T cells as a 70 kDa disulfide-linked heterodimer with CD94. It serves as a receptor for HLA-E (Qa-1b in mice), an MHC-I like molecule that largely presents fragments of signal peptides associated with classical MHC-I molecules. When engaged, NKG2H is potentially activating, and perhaps will initiate cytolysis and cytokine production. Alternatively, it has been suggested that NKG2H is involved in monitoring the physiological state of its target cell, presumably through the detection of increased levels of HLA-E. In either case, this activity is likely to be context-dependent, as the related NK2GA and B isoforms are inhibitory in nature, and compete for the same target. NKG2H is a 257 amino acid (aa) type II transmembrane glycoprotein. It contains a 70 aa cytoplasmic segment coupled to a 164 aa extracellular region (aa 94-257) that possesses a 108 aa C-type lectin domain (aa 117-224). NKG2H is the long isoform of the KLRC3 gene (SwissProt #:Q07444). The short form (NKG2E) is 240 aa in length, and diverges from the long form beginning at Val227 where 14 aa substitute for aa 227-257 of the long form. Over aa 241-253, human NKG2H shares no meaningful sequence identity with any other NKG2 isoforms or rodent species orthologs.

  • Long Name:
    Natural Killer G2H
  • Entrez Gene IDs:
    3823 (Human)
  • Alternate Names:
    KLRC3; NKG2H
Related Research Areas
Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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