Human NKp44/NCR2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp44/NCR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp46, and rhNKp80 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
    Gln22-Pro190, predicted
    Accession # O95944
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <1.5 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NKp44/NCR2 Fc Chimera (Catalog # 2249-NK)
  • Agonist Activity
    0.2-0.6 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Human NKp44 Antibody Induces IFN-gamma  Secretion in Human NK Cells.

Human NKp44 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in human natural killer (NK) cells activated with 1,000 U/mL Recombinant Human IL-2 (Catalog # 202-IL), in a dose-dependent manner, as measured using the Quant­ikine Human IFN‑ gamma  ELISA Kit (Catalog # DIF50). The ED50 for this effect is typically 0.2‑0.6 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp44/NCR2

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is up‑regulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
    4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
    5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  • Long Name:
    NK Cell Activating Receptor
  • Entrez Gene IDs:
    9436 (Human)
  • Alternate Names:
    CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKp44; NK-p44dJ149M18.1
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