Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 259415
View all NKX2.5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952

Specificity

Detects endogenous human NKX2.5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NKX2.5 Antibody

Detection of Human NKX2.5 antibody by Western Blot.

Detection of Human NKX2.5 by Western Blot.

Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human NKX2.5 antibody by Simple WesternTM.

Detection of Human NKX2.5 by Simple WesternTM.

Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human NKX2.5 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: CEM human T-lymphoblastoid cell line and iBJ6 human induced pluripotent stem cell linedifferentiated to cardiomyocytes

Western Blot

1 µg/mL
Sample: CEM human T-lymphoblastoid cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NKX2.5

NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart. NKX2.5 protein contains a central single DNA binding domain flanked by two Ala/Pro rich domains. Sumoylation of NKX2.5 at Lys-51 enhances its DNA binding and transcriptional activity.

Long Name

NK2 Homeobox 5

Alternate Names

CSX1, NKX2-5, NKX2E

Entrez Gene IDs

1482 (Human)

Gene Symbol

NKX2-5

UniProt

P52952

Additional NKX2.5 Products

Product Documents for Human NKX2.5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NKX2.5 Antibody

For research use only

Customer Reviews for Human NKX2.5 Antibody

There are currently no reviews for this product. Be the first to review Human NKX2.5 Antibody and earn rewards!

Have you used Human NKX2.5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies