Detection of Human NKX3.1 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NKX3.1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
NKX3.1 in LNCaP Human Cell Line.
NKX3.1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6080) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NKX3.1 (Homeobox protein NK-3 homolog A) is a 32-38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in testis and prostatic epithelium, and appears to be regulated by both testosterone and estrogen (but not progesterone). NKX3.1 apparently promotes prostate gland development, and acts as a tumor suppressor/transcriptional repressor by interacting with HDAC-1 and increasing p53 acetylation and half-life. Human NKX3.1 is 234 amino acids (aa) in length. It contains a DNA binding homeodomain (aa 124-183) and phosphorylation sites at Ser185 and Ser196 that regulate protein turnover. There are four splice variants that show changes N-terminal to the homeobox domain. Deletions of aa 8-56, 13-87, 15-83 and 40-83 are all reported. Over aa 1-123, human NKX3.1 shares 50% aa identity with mouse NKX3.1.
NK3 Homeobox 1
Entrez Gene IDs:
4824 (Human); 18095 (Mouse); 305999 (Rat)
BAPX2; BAPX2homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NK homeobox (Drosophila), family 3, A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1 (Drosophila); NK3 transcription factor related, locus 1; NKX3.1; NKX3.1NKX3; NKX3-1; NKX3A; NKX3ANK homeobox, family 3, A
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