Human NT-3 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF,
    rh beta ‑NGF, and rhNT-4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 41512
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3
    Tyr139-Thr257
    Accession # P20783
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human NT-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human NT‑3 Antibody (Catalog # MAB267)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human NT‑3 Biotinylated Antibody (Catalog # BAF267)
  • ELISA Standard
     
    Recombinant Human NT-3 Protein (Catalog # 267-N3)
  • Neutralization
    Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 100 ng/mL Recombinant Human NT‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. Recombinant Human NT‑3 (Catalog # 267-N3) stimulates proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT‑3 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human NT‑3 Monoclonal Antibody (Catalog # MAB267). The ND50 is typically
0.1‑0.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  • References:
    1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
    2. Snider, W.D. (1994) Cell 77:627.
    3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
  • Long Name:
    Neurotrophin 3
  • Entrez Gene IDs:
    4908 (Human)
  • Alternate Names:
    3-nucleotidase; NT3; NT-3; NTF3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. AAV1.NT-3 gene therapy for charcot-marie-tooth neuropathy.
    Authors: Sahenk Z, Galloway G, Clark K, Malik V, Rodino-Klapac L, Kaspar B, Chen L, Braganza C, Montgomery C, Mendell J
    Mol Ther, 2014;22(3):511-21.
    Species: Human
    Sample Type: Serum
    Application: ELISA Capture
  2. Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis.
    Authors: Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P
    J. Clin. Invest., 2010;120(3):850-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  3. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008;151(3):476-86.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
    Authors: Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC
    J. Exp. Med., 2003;198(3):455-67.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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