Human NT-3 Antibody

Catalog # Availability Size / Price Qty
MAB267-SP
MAB267-500
MAB267-100
Human NT-3 Antibody in Neutralization
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Product Details
Citations (4)
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Human NT-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF,
rh beta ‑NGF, and rhNT-4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 41512
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3
Tyr139-Thr257
Accession # P20783
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human NT-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human NT‑3 Biotinylated Antibody (Catalog # BAF267)

Standard: Recombinant Human NT-3 Protein (Catalog # 267-N3)

Neutralization
Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 100 ng/mL Recombinant Human NT‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. View Larger

Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human NT-3 Monoclonal Antibody (Catalog # MAB267). The ND50is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Neurotrophin 3
Entrez Gene IDs
4908 (Human)
Alternate Names
HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3

Product Datasheets

Citations for Human NT-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. AAV1.NT-3 gene therapy for charcot-marie-tooth neuropathy.
    Authors: Sahenk Z, Galloway G, Clark K, Malik V, Rodino-Klapac L, Kaspar B, Chen L, Braganza C, Montgomery C, Mendell J
    Mol Ther, 2014;22(3):511-21.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis.
    Authors: Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P
    J. Clin. Invest., 2010;120(3):850-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008;151(3):476-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
    Authors: Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC
    J. Exp. Med., 2003;198(3):455-67.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization

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