Human OAS2 Antibody (HLS56/3)

R&D Systems | Catalog # MAB1925

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HLS56/3
View all OAS2 Primary Antibodies »

Product Specifications

Immunogen

2’,5’-Oligoadenylate Synthetase-2

Specificity

Detects human OAS2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human OAS2 Antibody (HLS56/3)

OAS2 in U266 Human Cell Line.

OAS2 was detected in immersion fixed U266 human myeloma cell line using Mouse Anti-Human OAS2 Monoclonal Antibody (Catalog # MAB1925) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human OAS2 Antibody (HLS56/3)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed U266 human myeloma cell line

Immunoprecipitation

Hovanessian, A.G. et al. (1987) EMBO J. 6:1273.

Reviewed Applications

Read 1 review rated 5 using MAB1925 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OAS2

OAS2, also known as OAS (69 kDa), is one of three related proteins (along with OAS1 and OAS3) that catalyze the conversion of ATP into 2’,5’-linked adenosine oligomers with the general formula pppA(2’,5’A)n where n > 1 (2, 3) These proteins are induced by interferons, activated by double-stranded RNA, and are implicated in anti-viral immunity (1, 4, 5).

References

  1. Hovanessian, A.G. et al. (1987) EMBO J. 6:1273.
  2. Hovanessian, A.G. et al. (1977) Nature 268:537.
  3. Kerr, I.M. and R.E. Brown (1978) Proc. Natl. Acad. Sci. USA 75:256.
  4. Hovanessian, A.G. et al. (1988) J. Biol. Chem. 263:4959.
  5. Marie, I. et al. (1990) J. Interferon Res. 10:571.
  6. Marie, I. et al. (1990) J. Biol. Chem. 265:18601.

Long Name

2’,5’-Oligoadenylate Synthetase-2

Alternate Names

(2-5')oligo(A) synthase 2, (2'-5')oligo(A) synthetase 2, (2-5')oligo(A) synthetase 2, 2'5' oligoadenylate synthetase 2, 2-5A synthase 2, 2-5A synthetase 2, 2'-5'-oligoadenylate synthase 2, 2'-5'-oligoadenylate synthetase 2 (69-71 kD), 2'-5'-oligoadenylate synthetase 2, 69/71kDa, EC 2.7.7, EC 2.7.7.-, MGC78578, p69 OAS / p71 OAS, p69OAS / p71OAS

Entrez Gene IDs

4939 (Human)

Gene Symbol

OAS2

Additional OAS2 Products

Product Documents for Human OAS2 Antibody (HLS56/3)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human OAS2 Antibody (HLS56/3)

For research use only

Citations for Human OAS2 Antibody (HLS56/3)

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  • Human OAS2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Fibroblast lysate
    Species: Human
    Verified Customer | Posted 07/12/2021
    Human OAS2 Antibody (HLS56/3) MAB1925

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