Human OBCAM/OPCML Antibody

Catalog # Availability Size / Price Qty
AF2777
AF2777-SP
OBCAM/OPCML in MCF‑7 Human Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human OBCAM/OPCML Antibody Summary

Species Reactivity
Human
Specificity
Detects human OBCAM/OPCML in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) KILON is observed and less than 1% cross-reactivity with rhLAMP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human OBCAM/OPCML
Gly28-Asn322
Accession # Q14982
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human OBCAM (Catalog # 2777-CM)
Flow Cytometry
2.5 µg/106 cells
NG108‑15 mouse neuroblastoma/rat glioma hybrid cell line
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cortex)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry OBCAM/OPCML antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

OBCAM/OPCML in MCF‑7 Human Cell Line. OBCAM/OPCML was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human OBCAM/OPCML Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2777) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OBCAM/OPCML

OBCAM (Opioid-binding cell adhesion molecule, also known as OPCML) is a member of the IgLON family of cell adhesion molecules. All IgLONs are GPI-linked glycoproteins, contain three C2 type Ig-like domains, and are expressed in cerebral cortex and hippocampus (1, 2). The name IgLON derives from family membership in the Ig superfamily, and the first letters of the names of group’s molecules; LAMP, OBCAM, and Neurotrimin. Recently, membership in the group has been expanded by one with the addition of Kilon (Kindred of IgLON), and members of this group are now often referred to Diglons, based on the dimerizing nature of the IgLONs (1, 2). Human OBCAM is synthesized as a 345 amino acid (aa) preproprecursor that contains a 27 aa signal sequence, a 295 aa mature region, and a C‑terminal 23 aa prosegment (3). The prosegment is cleaved to generate the GPI-link. OBCAM varies in molecular weight, ranging from 46 kDa to 65 kDa (4‑6). The difference is not due to alternate splicing but to differential glycosylation (6). Although it is not unusual for GPI-linked proteins to be solubilized, to date there is no evidence that OBCAM functions as a soluble molecule (1). Mature human OBCAM is 98%, 99%, and 98% aa identical to mature bovine, rat and mouse OBCAM, respectively. OBCAM has limited expression, occurring principally in telencephalon and ovarian epithelium (7, 8). In brain, it is found associated with dendrites and post-synaptic membranes, where it may maintain synaptic architecture (1, 5). In ovary, it has been suggested to be a tumor-suppressor factor (8). The receptor(s) for OBCAM appears to be other members of the IgLON family, and a dimer is the functional unit. While neurotrimin appears to function as both a homodimer and heterodimer, all other family members (including OBCAM) show a preference for heterodimerization. OBCAM forms strong trans (between cells) heterodimers with LAMP, and modest heterodimers with Neurotrimin. There is hardly any binding with itself. Kilon likely binds OBCAM, but this interaction is not well studied (1). OBCAM heterodimers apparently bind to almost all possible IgLON heterodimer combinations on other cells. In cis (same cell), OBCAM also binds to LAMP and Neurotrimin (2).

References
  1. Miyata, S. et al. (2000) Neuroscience 117:645.
  2. Reed, J. et al. (2004) J. Cell Sci. 117:3961.
  3. Shark, K.B. and N.M. Lee (1995) Gene 155:213.
  4. Wick, M.J. et al. (1996) Mol. Brain Res. 36:322.
  5. Miyata, S. (2000) J. Comp. Neurol. 242:74.
  6. Hachisuka, A. et al. (1996) Neurochem. Int. 28:373.
  7. Miyata, S. et al. (2003) Brain Res. 979:129.
  8. Sellar, G.C. et al. (2003) Nat. Genet. 34:337.
Long Name
Opioid-binding Cell Adhesion Molecule/Opioid Binding Protein/Cell Adhesion Molecule-like
Entrez Gene IDs
4978 (Human); 330908 (Mouse)
Alternate Names
IgLON family member 1; IGLON1opioid-binding protein/cell adhesion molecule-like; OBCAM; OBCAMopiate binding-cell adhesion molecule; OPCM; OPCML; opioid binding protein/cell adhesion molecule-like preprotein; opioid binding protein/cell adhesion molecule-like; Opioid-binding cell adhesion molecule; opioid-binding protein/cell adhesion molecule

Product Datasheets

Citation for Human OBCAM/OPCML Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions
    Authors: JR Birtley, M Alomary, E Zanini, J Antony, Z Maben, GC Weaver, C Von Arx, M Mura, AT Marinho, H Lu, EVN Morecroft, E Karali, NE Chayen, EW Tate, M Jurewicz, LJ Stern, C Recchi, H Gabra
    Nat Commun, 2019;10(1):3134.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human OBCAM/OPCML Antibody

There are currently no reviews for this product. Be the first to review Human OBCAM/OPCML Antibody and earn rewards!

Have you used Human OBCAM/OPCML Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review