Human Osteoadherin/OSAD Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Osteoadherin/OSAD in ELISA.
  • Source
    Monoclonal Mouse IgG2B Clone # 806001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human Osteoadherin/OSAD
    Gln21-Gln421
    Accession # Q99983
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Osteoadherin/OSAD in Human Mesenchymal Stem Cells. Osteoadherin/OSAD was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human Osteoadherin/OSAD Monoclonal Antibody (Catalog # MAB2884) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoadherin/OSAD

Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20‑30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix (1, 2). The human OSAD cDNA encodes a 421 aa precursor that contains a 20 aa signal sequence and eleven tandem leucine rich repeats (3). Human OSAD shares 80‑84% aa sequence identity with bovine, mouse, and rat OSAD. Human OSAD shares 32‑35% aa sequence identity with human class II SLRPs Fibromodulin, Keratocan, Lumican, and PRELP. Bovine, mouse, and rat OSAD are expressed as 60‑85 kDa molecules, although the amino acid sequence for each predicts a size of 46‑47 kDa. The primary difference is due to the presence of extensive N-linked glycosylation that can also vary between tissues of the same species (4, 5). Human OSAD is expressed as an even larger 110 kDa molecule in teeth (6). OSAD contains eight sulfated tyrosine residues (4, 7) and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C-terminal acidic domain (3). OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon (3, 4, 8). In dental tissue, OSAD is expressed by odontoblasts and ameloblasts (5, 9‑11) and is involved in the mineralization of bone and teeth (5, 11, 12). OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3 (4, 6).

  • References:
    1. Matsushima, N. et al. (2000) Proteins 38:210. 
    2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725. 
    3. Sommarin, Y. et al. (1998) J. Biol. Chem. 273:16723. 
    4. Wendel, M. et al. (1998) J. Cell Biol. 141:839. 
    5. Hultenby, P.U. et al. (2003) Eur. J. Oral Sci. 111:128. 
    6. Lucchini, M. et al. (2004) J. Dent. Res. 83:552. 
    7. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26
    8. Shen, Z. et al. (1999) Matrix Biol. 18:533.
    9. Buchaille, R. et al. (2000) Bone 27:265.
    10. Buchaille, R. et al. (2000) Matrix Biol. 19:421.
    11. Couble, M.L. et al. (2004) Histochem. Cell Biol. 121:47.
    12. Ramstad, V.E. et al. (2003) Calcif. Tissue Int. 72:57.
  • Entrez Gene IDs:
    4958 (Human)
  • Alternate Names:
    Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; OMD; OSAD; osteoadherin proteoglycan; Osteoadherin; osteomodulin; SLRR2C; SLRR2Costeoadherin
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