Human P-Rex1 Antibody

Catalog # Availability Size / Price Qty
MAB8076
MAB8076-SP
Detection of Human P-Rex1 by Simple WesternTM.
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Human P-Rex1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human P-Rex1 in ELISAs and Western Blots.
Source
Monoclonal Mouse IgG2B Clone # 869704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human P-Rex1
Lys1313-Arg1434
Accession # Q8TCU6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Simple Western Detection of Human P-Rex1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human P-Rex1 by Simple WesternTM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for P-Rex1 at approximately 175 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human P-Rex1 antibody by Western Blot. View Larger

Detection of Human P-Rex1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Rex1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Rex1

PREX1 (phosphoinositol (3,4,5)-dependent Rac exchanger 1), also called P-Rex1, is a 185 kDa G-protein-activated guanine nucleotide exchange factor. It is mainly cytosolic, but also plasma membrane-associated, regulating cell motility and actin dynamics in neutrophils, migrating neurons, adipocytes and platelets. It is upregulated in cancers such as melanoma, prostate and breast. The 1659 amino acid (aa) human P-Rex1 contains DH, PH and tandem DEP GPCR signaling domains, and a PDZ scaffolding domain between aa 49-703. A 956 aa isoform with a 5 aa substitution for aa 1-703 is reported, and a 1561 aa isoform with a 91 aa substitution for aa 1471-1659 is predicted. The human P-Rex1 immunogen (aa 1313-1434) shares 94% and 95% aa sequence identity with mouse and rat P-Rex1, respectively.

Long Name
Phosphatidylinositol-3,4,5-Trisphosphate-dependent Rac Exchange Factor 1
Entrez Gene IDs
57580 (Human); 277360 (Mouse); 311647 (Rat)
Alternate Names
KIAA1415; phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein; phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1; PRex1; P-Rex1; ptdIns(34,5)-dependent Rac exchanger 1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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