Human P-Rex1 Antibody

Catalog # Availability Size / Price Qty
MAB8076
MAB8076-SP
Human P-Rex1 Antibody in Western Blot
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Human P-Rex1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human P-Rex1 in ELISAs and Western Blots.
Source
Monoclonal Mouse IgG2B Clone # 869704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human P-Rex1
Lys1313-Arg1434
Accession # Q8TCU6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human P-Rex1 by Western Blot. View Larger

Detection of Human P-Rex1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Rex1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human P-Rex1 by Simple Western<sup>TM</sup>. View Larger

Detection of Human P-Rex1 by Simple WesternTM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for P-Rex1 at approximately 175 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Rex1

PREX1 (phosphoinositol (3,4,5)-dependent Rac exchanger 1), also called P-Rex1, is a 185 kDa G-protein-activated guanine nucleotide exchange factor. It is mainly cytosolic, but also plasma membrane-associated, regulating cell motility and actin dynamics in neutrophils, migrating neurons, adipocytes and platelets. It is upregulated in cancers such as melanoma, prostate and breast. The 1659 amino acid (aa) human P-Rex1 contains DH, PH and tandem DEP GPCR signaling domains, and a PDZ scaffolding domain between aa 49-703. A 956 aa isoform with a 5 aa substitution for aa 1-703 is reported, and a 1561 aa isoform with a 91 aa substitution for aa 1471-1659 is predicted. The human P-Rex1 immunogen (aa 1313-1434) shares 94% and 95% aa sequence identity with mouse and rat P-Rex1, respectively.

Long Name
Phosphatidylinositol-3,4,5-Trisphosphate-dependent Rac Exchange Factor 1
Entrez Gene IDs
57580 (Human); 277360 (Mouse); 311647 (Rat)
Alternate Names
KIAA1415; phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein; phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1; PRex1; P-Rex1; ptdIns(34,5)-dependent Rac exchanger 1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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