Biologic responses to ATP, ADP, and other extracellular nucleotides are mediated by P2-nucleotide receptors belonging to 2 major classes: G protein-coupled P2Y receptors and nucleotide-gated ion channel P2X receptors. P2RX1 functions as a ligand-gated ion channel with relatively high calcium permeability. Mouse studies suggest that this receptor is essential for normal male reproductive function. The development of selective antagonists for this receptor may provide an effective non-hormonal male contraceptive pill.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 551820
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Product Specifications
Immunogen
Human embryonic kidney cell, HEK293-derived transfected with human P2X1/P2RX1
Accession # P51575
Accession # P51575
Specificity
Detects human P2X1/P2RX1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human P2X1/P2RX1 Antibody
Detection of P2X1/P2RX1 in HEK293 Human Cell Line transfected with Human P2X1/P2RX1 and eGFP by Flow Cytometry
HEK293 human embryonic kidney cell line transfected with either (A) human P2X1/P2RX1 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human P2X1/P2RX1 monoclonal antibody (Catalog # MAB10533) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.Applications for Human P2X1/P2RX1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with Human P2X1/P2RX1 and eGFP
Sample: HEK293 Human Cell Line transfected with Human P2X1/P2RX1 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: P2X1/P2RX1
Long Name
P2X Purinergic Receptor 1
Alternate Names
P2RX1, P2XMR
Gene Symbol
P2RX1
UniProt
Additional P2X1/P2RX1 Products
Product Documents for Human P2X1/P2RX1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human P2X1/P2RX1 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
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- Flow Cytometry Protocol for Staining Membrane Associated Proteins
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- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
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- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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