Human Pancreatic Polypeptide/PP Antibody

Catalog # Availability Size / Price Qty
Pancreatic Polypeptide/PP in Human Pancreas.
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Human Pancreatic Polypeptide/PP Antibody Summary

Species Reactivity
Detects human Pancreatic Polypeptide/PP in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 548416
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Pancreatic Polypeptide/PP
Accession # P01298
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Pancreatic Polypeptide/PP antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Pancreatic Polypeptide/PP in Human Pancreas. Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Pancreatic Polypeptide/ PP Monoclonal Antibody (Catalog # MAB62971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pancreatic Polypeptide/PP

Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence (1). PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake (2). The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

  1. Leiter A, et al. (1984) J. Biol. Chem. 259:14702.
  2. Asakawa, A et al. (2003) Gastroenterology 124:1325.
Entrez Gene IDs
5539 (Human)
Alternate Names
INN; Obinepitide; pancreatic polypeptide (ppy); pancreatic polypeptide PNPPP; pancreatic polypeptide Y; Pancreatic Polypeptide; pancreatic prohormone; PNP; PP; PPY

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Human Pancreatic Polypeptide/PP Antibody
By Anonymous on 02/26/2022
Application: IHC Sample Tested: Adult pancreas Species: Human