Human PD-ECGF/Thymidine Phosphorylase Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PD‑ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD‑ECGF/Thymidine Phosphorylase
    Ala11-Gln482
    Accession # P19971
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.01 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229-PE)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize PD‑ECGF/Thymidine Phosphorylase-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF/Thymidine Phosphorylase.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.

Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229‑PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD‑ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Human PD‑ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143). The ND50 is typically 0.5-2.0 µg/mL.

Immunohistochemistry
PD‑ECGF/Thymidine Phosphorylase in Human Prostate Cancer Tissue. PD‑ECGF/Thymidine Phosphorylase was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human PD‑ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-ECGF/Thymidine Phosphorylase
PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit 3H-thymidine incorporation.
  • Long Name:
    Platelet-derived Endothelial Cell Growth Factor
  • Entrez Gene IDs:
    1890 (Human); 72962 (Mouse)
  • Alternate Names:
    dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP
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