Human PDCD4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PDCD4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 808508
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PDCD4
    Lys212-His358
    Accession # Q53EL6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
PDCD4 in MCF‑7 Human Cell Line. PDCD4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human PDCD4 Monoclonal Antibody (Catalog # MAB7019) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDCD4

PDCD4 (Programmed cell death protein 4; also H731) is a 54-64 kDa member of the PDCD4 family of molecules. It is widely expressed, being found in mammary epithelium, CD34+ bone marrow progenitor cells, fibroblasts and keratinocytes. PDCD4 is both cytoplasmic and nuclear. In the cytoplasm, it blocks protein translation by binding to eIF4A, an act that dissociates eIF4G and mRNA from eIF4A. In the nucleus, it seems to block transcription of select genes, one of which is MAP4K1, a key enzyme in the AP-1-mediated transcription pathway. Human PDCD4 is 469 amino acids (aa) in length. It contains an NLS (aa 58-64), an MI domain (aa 163-284), another NLS (aa 241-250) and a second MI domain (aa 326-449). There are at least seven utilized Ser/Thr phosphorylation sites and one Tyr phosphorylation site. There are two potential splice forms, one that contains a deletion of aa 15-28, and a second that may show a three aa substitution for aa 2-15. Over aa 212-357, human PDCD4 shares 98% aa identity with mouse PDCD4.

  • Long Name:
    Programmed Cell Death 4
  • Entrez Gene IDs:
    27250 (Human); 18569 (Mouse); 64031 (Rat)
  • Alternate Names:
    H731; H731nuclear antigen H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a
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