Human PDGF-C Antibody

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Detection of Human PDGF‑C by Western Blot.
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Product Details
Citations (4)
Supplemental Products

Human PDGF-C Antibody Summary

Species Reactivity
Detects human PDGF-C in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPDGF‑AB, and rhPDGF‑D is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PDGF-C
Accession # Q9NRA1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
Measured by its ability to neutralize PDGF‑CC-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 0.8 µg/mL Recombinant Human PDGF‑CC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PDGF-C antibody by Western Blot. View Larger

Detection of Human PDGF‑C by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry PDGF‑C antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDGF‑C in Human Pancreatic Cancer Tissue. PDGF‑C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 µg/mL Goat Anti-Human PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. View Larger

Cell Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. Recombinant Human PDGF-CC (Catalog # 1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND50 is typically 6-24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-C

Platelet-derived growth factor C (PDGF-C), also named spinal cord derived growth factor (SCDGF) and follotain, is a member of the PDGF family that binds to the PDGF receptor alpha alpha and alpha beta. PDGF-C is a growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is a potent mitogen and chemoattractant for cells of mesenchymal origin. It is also required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. In addition, PDGF-C is required for normal skin morphogenesis during embryonic development. PDGF-C plays an important role in angiogenesis and blood vessel development and is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Western blot analysis of platelets identified 55 kDa and 80 kDa PDGF C forms that were secreted on platelet activation (1).

Fang L. et al. (2004) Arterioscler Thromb Vasc Biol. 24:787
Long Name
Platelet-derived Growth Factor C
Entrez Gene IDs
56034 (Human); 54635 (Mouse)
Alternate Names
FALLOTEIN; PDGFC; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E

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Citations for Human PDGF-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
    Authors: T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto
    Cancer Research, 2023-03-02;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016-11-22;7(47):77902-77915.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
    Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
    Oncol Rep, 2013-10-10;30(6):2887-96.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Functional Assay, Western Blot
  4. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
    Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
    Atherosclerosis, 2008-05-22;202(2):415-23.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: IHC-Fr, Neutralization, Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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